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HTRF Mouse IL-1α Detection Kit, 500 Assay Points
HTRF Mouse IL-1α Detection Kit, 500 Assay Points
PHOTO MOUSE IL1-ALPHA KIT
Mouse IL1-α kit is designed quantification of mouse IL1-α release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 16 µL |
Mouse IL1-α kit is designed quantification of mouse IL1-α release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Unit Size: 500 Assay Points
Part #:
62MIL1APEG
List Price
USD 1,103.00
Unit Size: 10,000 Assay Points
Part #:
62MIL1APEH
List Price
USD 10,290.00
HTRF Mouse IL-1α Detection Kit, 500 Assay Points
PHOTO MOUSE IL1-ALPHA KIT
HTRF Mouse IL-1α Detection Kit, 500 Assay Points
Product information
Overview
Also known as hematopoietin 1, IL1 alpha is a pro-inflammatory cytokine mainly produced by activated macrophages, and epithelial and endothelial cells. Its main role is the maintenance and protection of the skin's barrier function. In response to infection, IL1 alpha stimulates fibroblast proliferation, increases blood neutrophils, and activates lymphocyte proliferation.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Cytokines
|
| Target Species |
Mouse
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Oncology & Inflammation
|
| Unit Size |
500 Assay Points
|
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HTRF Mouse IL-1α Detection Kit, 500 Assay Points
HTRF Mouse IL-1α Detection Kit, 500 Assay Points
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Assay details
Technical specifications of mouse IL1 alpha kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 34 pg/mL &62 pg/mL |
| Range | 62 4,000 pg/mL |
| Time to result | 3h at RT |
| Calibration | NIBSC (93/672) value (IU/mL) = 1,2 x HTRF mIL1a value (pg/mL) |
| Species | Mouse only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [IL1a] (pg/mL) | CV |
|---|---|---|
| 1 | 100 | 11% |
| 2 | 800 | 3% |
| 3 | 3200 | 1% |
| Mean CV | 5% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [IL1a] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 161 | 188 | 3% |
| 2 | 583 | 595 | 2% |
| 3 | 2105 | 1674 | 2% |
| Mean CV | 2% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Resources
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