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HTRF Mouse IFNγ Detection Kit, 10,000 Assay Points
The HTRF mouse IFN gamma kit is designed for the quantification of mouse IFN gamma release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
IFN gamma is a pro-inflammatory cytokine secreted by T and NK cells in response to viral infection and or inflammatory conditions. IFN gamma plays a major role in innate and adaptive immunity by promoting T cell differentiation into TH1 and development of Treg, but also by inhibiting T cell differentiation into TH17. Its anti-tumor properties have been used in treatment of osteoporosis and it is of great interest in immuno-oncology, where it improves survival in patients with bladder or ovarian carcinoma.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.
Specifications
| Assay Points |
10000
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Therapeutic Area |
Oncology & Inflammation
|
| Unit Size |
10,000 Assay Points
|
Video gallery
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting,
Revvity has also worked with Myassays.com to help you in your data analysis, you’ll be able to access a free online software to run your IFN gamma analysis.
Assay details
Technical specifications of mouse IFN gamma kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 23 pg/mL &34 pg/mL |
| Range | 34 3,000 pg/mL |
| Time to result | 2h at RT |
| Species | Mouse only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [IFNg] (pg/mL) | CV |
|---|---|---|
| 1 | 80 | 11% |
| 2 | 1375 | 2% |
| 3 | 2750 | 2% |
| Mean CV | 5% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [IFNg] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 94 | 213 | 5% |
| 2 | 375 | 868 | 10% |
| 3 | 1500 | 3502 | 13% |
| Mean CV | 9% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Resources
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
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