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HTRF Mouse Fc Kit, 500 Assay Points
The Mouse Fc kit is designed for the fast quantification of mouse IgG or mouse Fc tagged proteins.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The Mouse Fc kit enables a rapid quantification of mouse IgG, and any proteins containing a mouse Fc region. The assay is compatible with natural IgG as well as recombinant antibodies.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Rare Diseases
|
| Unit Size |
500 Assay Points
|
Video gallery
How it works
Assay principle
The mouse Fc kit is a competitive immunoassay for the measurement of mIgGs or any mFc-tagged proteins in a range of biological materials. The assay uses an Eu3+-Cryptate conjugated antibody specific to mFc and XL665 coupled mIgGs. mFc contained in the sample competes with XL665-labelled mouse IgG for binding to anti-mouse Fc Eu Cryptate.
Assay Protocol
The mouse Fc assay features a streamlined protocol with only 1 incubation step after sample and Mouse Fc detection reagents dispensing. This protocol requires a single two-hour incubation period.
Assay details
Key features
| Mouse Fc | |
| Detection limit: | 6.8 ng/mL |
| Dynamic range: | 0.8 400,000 ng/mL |
| Incubation time: | 2h at RT |
| EC50: | 138 ng/mL |
Resources
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
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