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HTRF Mouse IgG-Fc Detection Kit, 10,000 Assay Points

The Mouse Fc kit is designed for the fast quantification of mouse IgG or mouse Fc tagged proteins.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Protein Quantification
Sample Volume 10 µL

The Mouse Fc kit is designed for the fast quantification of mouse IgG or mouse Fc tagged proteins.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Unit Size: 500 Assay Points
Part #:
6FMIGPEG
List Price
USD 778.36
Unit Size: 10,000 Assay Points
Part #:
6FMIGPEH
List Price
USD 5,350.00

Overview

Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The Mouse Fc kit enables a rapid quantification of mouse IgG, and any proteins containing a mouse Fc region. The assay is compatible with natural IgG as well as recombinant antibodies.

Specifications

Application
Protein Quantification
Brand
HTRF
Detection Modality
HTRF
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Dry Ice
Target Class
Biologics
Target Species
Mouse
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Rare Diseases
Unit Size
10,000 Assay Points

Video gallery

How it works

Assay principle

The mouse Fc kit is a competitive immunoassay for the measurement of mIgGs or any mFc-tagged proteins in a range of biological materials. The assay uses an Eu3+-Cryptate conjugated antibody specific to mFc and XL665 coupled mIgGs. mFc contained in the sample competes with XL665-labelled mouse IgG for binding to anti-mouse Fc Eu Cryptate.

Biomarkers assay principle mouse
Assay Protocol

The mouse Fc assay features a streamlined protocol with only 1 incubation step after sample and Mouse Fc detection reagents dispensing. This protocol requires a single two-hour incubation period.

Biomarkers assay protocol mouse

 

Assay details

Key features
  Mouse Fc
Detection limit: 6.8 ng/mL
Dynamic range: 0.8  400,000 ng/mL
Incubation time: 2h at RT
EC50: 138 ng/mL
Specifications mouse

 

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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