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HTRF mAb Anti-Phospho-AKT (Ser/Thr) Eu-Conjugate, 500 Assay Points

MAb Anti-phospho AKT-Eu cryptate enables the rapid detection of Akt phosphorylation on Ser473 in a biochemical kinase assay format.

For research use only. Not for use in diagnostic procedures.

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Feature	Specification
Application	Biochemical Enzymatic Assay

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MAb Anti-phospho AKT-Eu cryptate enables the rapid detection of Akt phosphorylation on Ser473 in a biochemical kinase assay format.

For research use only. Not for use in diagnostic procedures.

View product information

Product Variants

Unit Size: 500 Assay Points

Part #:

61P15KAE

List Price

USD 844.44

Unit Size: 10,000 Assay Points

Part #:

61P15KAZ

List Price

USD 2,285.00

Product information

Overview

The Anti-phospho AKT-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of Akt only when phosphorylated at Ser473. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.

Specifications

Other Specifications

Application	Biochemical Enzymatic Assay
Brand	HTRF
Detection Modality	HTRF
Molecular Modification	Phosphorylation
Product Group	Fluorescent Reagent
Shipping Conditions	Shipped in Dry Ice
Target Class	Kinase
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Inflammation Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	500 Assay Points

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How it works

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

HTRF MAb Anti-phospho AKT-Eu cryptate 1.svg

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phospho AKT-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.