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HTRF LTC4 Detection Kit, 500 Assay Points

The Leukotriene C4 kit is designed for the accurate quantification of LTC4.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein Quantification
Sample Volume 10 µL

The Leukotriene C4 kit is designed for the accurate quantification of LTC4.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
64LC4PEG
List Price
USD 1,039.00
Unit Size: 10,000 Assay Points
Part #:
64LC4PEH
List Price
USD 11,540.00

Overview

LTC4 is a major leukotriene product that is enzymatically derived from the arachidonic acid cascade. Leukotriene synthesis operates via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The conjugation of glutathione to LTA4 results in the formation of LTC4, which is then rapidly metabolized to LTD4 and LTE4. LTC4 has been described as a potent mediator of inflammatory diseases and immunoregulation. The LTC4 kit enables the monitoring of LTC4 produced by cells and can be used to study the LTC4-hydrolase.

Specifications

Application
Protein Quantification
Brand
HTRF
Detection Modality
HTRF
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Dry Ice
Target Class
Biomarkers
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Neuroscience
Oncology & Inflammation
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

The LTC4 kit is a competitive immunoassay for the measurement of LTC4 in a range of biological formats. The assay uses an Eu3+-Cryptate conjugated antibody specific to LTC4 and XL665 coupled LTC4. LTC4 contained in the sample competes with XL665-labelled LTC4 for binding to anti-LTC4 Eu Cryptate.

Biomarkers how it works assay principle ltb4 64lc4peg 64lc4peh
Assay protocol

The LTC4 assay features a streamlined protocol with only 1 incubation step after sample and LTC4 detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.

Biomarkers how it works assay protocol ltb4 64lc4peg 64lc4peh

 

Assay details

Key features
  LTC4
Incubation 1h at RT
Assay range 0.1 5000 ng/mL
EC50 2.7 ng/mL
Detection limit 0.1 ng/mL
Specificity No cross reactivity with LTA4, LTB4

 

64LC4PEG specifications ltc4-1

 

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