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HTRF Leukotriene B4 Detection Kit, 500 Assay Points
The Leukotriene B4 kit is designed for the accurate quantification of LTB4.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
LTB4 is a major leukotriene product that is enzymatically derived from the arachidonic acid cascade. LTB4 is synthesized by leukocytes via the 5-lipoxygenase pathway and mediates different leukocyte functions such as lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been described as a potent mediator of inflammatory diseases and immunoregulation. The LTB4 kit enables the monitoring of LTB4 produced by cells and can be used to study the LTA4-hydrolase.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Therapeutic Area |
Cardiovascular
Neuroscience
Oncology & Inflammation
|
| Unit Size |
500 Assay Points
|
Video gallery
How it works
Assay principle
The LTB4 kit is a competitive immunoassay for the measurement of LTB4 in a range of biological formats. The assay uses an Eu3+-Cryptate conjugated antibody specific to LTB4 and XL665 coupled LTB4. LTB4 in the sample competes with XL665-labelled LTB4 for binding to anti-LTB4 Eu Cryptate.
Assay protocol
The LTB4 assay features a streamlined protocol with only 1 incubation step after sample and LTB4 detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.
Assay details
Key features
| LTB4 | |
| Incubation | 1h at RT |
| Assay range | 0.15 166.67 ng/mL |
| EC50 | 1.12 ng/mL |
| Detection limit | 0.15 ng/mL |
| Specificity | Low cross reactivity with 6-trans 12 epi LTB4 |
Resources
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
Flyer
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SDS, COAs, Manuals and more
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