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HTRF KinEASE STK-S2 Kit, 20,000 Assay Points

The HTRF KinEASE assay series is a semi-universal biochemical platform to study kinase activity. The STK2 kit uses a specific substrate compatible with various Serine/thronine kinases - cf. validation data provided on this page.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature	Specification
Application	Biochemical Enzymatic Assay
Sample Volume	10 µL

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Product Variants

Unit Size: 1,000 Assay Points

Part #:

62ST2PEB

List Price

USD 1,107.00

Unit Size: 20,000 Assay Points

Part #:

62ST2PEC

List Price

USD 7,343.00

Product information

Overview

The HTRF KinEASE kit series provides a simple biochemical approach to study kinase activity, screen for inhibitors and characterize them. Three STK and one TK kits, offer a semi-universal method for assessing phosphorylation on Serine/Threonine and Tyrosine residues, respectively. The HTRF KinEASE STK S2 assay has been validated on 18 Serine/Threonine kinases. The assay validation section of this page lists them and provides technical tips for setup. The kit series also include a Discovery kit to help determine which of three STK kits is appropriate when working with a non-validated enzyme.

Specifications

Other Specifications

Application	Biochemical Enzymatic Assay
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped Ambient
Target Class	Kinases
Technology	TR-FRET
Therapeutic Area	Metabolism/Diabetes NASH/Fibrosis Oncology & Inflammation
Unit Size	20,000 Assay Points

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How it works

Assay principle

Each HTRF KinEASE kit is based on the same assay format, and uses a universal biotinylated substrate, a phospho-specific Eu-cryptate-labeled monoclonal antibody, and SA-XL665. All three STK peptidic substrates are made of the same sequence for antibody detection, next to a variable sequence recognized by enzymes. The targeted residue for phosphorylation lies in between as part of both sequences for enzymatic activity (unphosphorylated) and antibody detection (phosphorylated). It has been shown that 3 different variable sequences could enable the study of most Serine/Threonine kinases.

Assay protocol

As shown here, all HTRF KinEASE assays work the same way and involve two steps:

Step 1: Kinase reaction The kinase is incubated in the presence or absence of compounds and the appropriate substrate. The supplemented enzymatic buffer is added in the kinase buffer for tyr kinases only. ATP is added to start the reaction.

Step 2: Detection Eu-cryptate and XL665 conjugates are added. The detection buffer contains EDTA to stop the enzymatic reaction.