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HTRF KinEASE-STK S2 Detection Kit, 1,000 Assay Points
The HTRF KinEASE assay series is a semi-universal biochemical platform to study kinase activity. The STK2 kit uses a specific substrate compatible with various Serine/thronine kinases - cf. validation data provided on this page.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
The HTRF KinEASE kit series provides a simple biochemical approach to study kinase activity, screen for inhibitors and characterize them. Three STK and one TK kits, offer a semi-universal method for assessing phosphorylation on Serine/Threonine and Tyrosine residues, respectively. The HTRF KinEASE STK S2 assay has been validated on 18 Serine/Threonine kinases. The assay validation section of this page lists them and provides technical tips for setup. The kit series also include a Discovery kit to help determine which of three STK kits is appropriate when working with a non-validated enzyme.
Specifications
| Assay Points |
1000
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped Ambient
|
| Therapeutic Area |
Metabolism/Diabetes
NASH/Fibrosis
Oncology & Inflammation
|
| Unit Size |
1,000 Assay Points
|
Video gallery
Citations
How it works
Assay principle
Each HTRF KinEASE kit is based on the same assay format, and uses a universal biotinylated substrate, a phospho-specific Eu-cryptate-labeled monoclonal antibody, and SA-XL665. All three STK peptidic substrates are made of the same sequence for antibody detection, next to a variable sequence recognized by enzymes. The targeted residue for phosphorylation lies in between as part of both sequences for enzymatic activity (unphosphorylated) and antibody detection (phosphorylated). It has been shown that 3 different variable sequences could enable the study of most Serine/Threonine kinases.
Assay protocol
As shown here, all HTRF KinEASE assays work the same way and involve two steps:
Step 1: Kinase reaction The kinase is incubated in the presence or absence of compounds and the appropriate substrate. The supplemented enzymatic buffer is added in the kinase buffer for tyr kinases only. ATP is added to start the reaction.
Step 2: Detection Eu-cryptate and XL665 conjugates are added. The detection buffer contains EDTA to stop the enzymatic reaction.
Resources
Literature - Publication Review
Download your literature review on the multiple use of KinEase assay kits
Discover the cutting edge of kinase assay knowledge
This literature review illustrates the possibilities and versatility of HTRF™...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
Guide
The ultimate guide to successful kinase binding experiments
Your guide for mastering the art of kinase binding assay optimizations
This complete guide provides you with all the tools you need...
Product Info
Validated kinases and assay conditions
The assay conditions used for kinases validated with HTRF KinEASE technology.
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
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