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HTRF Human IgG Kappa MAb Detection Kit, 500 Assay Points
The Human IgG Kappa MAb kit is designed for the fast quantification of human IgGs Kappa light chains.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The human IgG Kappa MAb kit enables a rapid quantification of human IgG Kappa light chains in cell supernatant. The assay is compatible with natural IgG as well as recombinant antibodies.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Rare Diseases
|
| Unit Size |
500 Assay Points
|
Video gallery
How it works
Assay principle
The human IgG Kappa MAb kit is detected in a sandwich assay format using 2 different specific antibodies. The anti-(h)-IgG Kappa antibody is labelled with d2 (acceptor) and the anti(h)-Fc antibody is labelled with Eu3+-Cryptate (donor). The two antibodies bind to the (h)IgG Kappa present in the sample, thereby generating FRET. The specific signal modulates positively in proportion to (h)IgG Kappa.
Assay protocol
The human IgG Kappa MAb assay features a streamlined protocol with only 1 incubation step after sample and IgG Kappa MAb detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.
Assay details
Key features
| Detection limit | 0.8 ng/mL |
| Dynamic range | 0.8 to 100 ng/mL |
| Specificity | subclasses IgG1, IgG2, IgG3 & IgG4 |
| Cross reactivity | 0% Human Lambda |
| 0% Mouse Kappa | |
| <1% Human IgM |
Resources
Whitepaper
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SDS, COAs, Manuals and more
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