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HTRF Human SMN Detection Kit, 500 Assay Points

This HTRF kit is designed for the rapid detection of human SMN produced by cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature	Specification
Application	Protein Quantification
Sample Volume	10 µL

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This HTRF kit is designed for the rapid detection of human SMN produced by cells.

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Product Variants

Unit Size: 500 Assay Points

Part #:

63ADK033PEG

List Price

USD 1,039.00

Unit Size: 10,000 Assay Points

Part #:

63ADK033PEH

List Price

USD 11,750.00

Product information

Overview

SMN (Survival of motor neuron) is a 38-kD protein that is ubiquitously expressed in both the cytoplasm and nucleus. SMN plays an important role in assembling snRNP particles and is involved in RNA editing and transport. A lower expression of SMN has been correlated to the onset of Spinal Muscular Atrophy (SMA), an autosomal recessive disorder that is the leading hereditary cause of infant mortality, characterized by a loss of motoneurons in the spinal cord. The human SMN kit is designed for the detection of survival motor neuron protein directly in cell lysates.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Biomarkers
Target Species	Human
Technology	TR-FRET
Therapeutic Area	Neuroscience Rare Diseases
Unit Size	500 Assay Points

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How it works

Assay principle

Human SMN is measured using a sandwich immunoassay involving two specific human SMN antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of SMN present in the sample.

Assay protocol

The Human SMN assay features a two-plate assay protocol, where cells are plated, stimulated, and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of SMN. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.