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HTRF Human MANF Detection Kit, 500 Assay Points
The human MANF kit is designed for the rapid quantification of Mesencephalic Astrocyte-derived Neutrophic Factor in cell supernatants and serum.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Mesencephalic astrocyte-derived neurotrophic factor (MANF) (also recognized as ARMET or arginine-rich mutated in early stages of tumors) belongs to the fourth family of neurotrophic factors. MANF is an endoplasmic reticulum (ER) resident protein highly expressed in the developing cortex that plays an important role in protecting and repairing dopaminergic neurons in the rat 6-hydroxydopamine Parkinson disease model. MANF is also involved in protecting against various forms of endoplasmic reticulum stress. The human MANF kit enables the fast detection of MANF in cell supernatants and serum.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Therapeutic Area |
Neuroscience
|
| Unit Size |
500 Assay Points
|
Video gallery
Citations
How it works
Assay principle
Human MANF is measured using a sandwich immunoassay involving two specific human MANF antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of MANF present in the sample.
Assay protocol
The MANF assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires a single 4-hour incubation period at RT.
Resources
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
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