The HTRF human IL5 kit is designed for the quantification of human IL5 release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein Quantification |
Sample Volume | 16 µL |
The HTRF human IL5 kit is designed for the quantification of human IL5 release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
IL5 is mainly produced by TH2 cells upon IL4 activation, but also by eosinophils and mast cells. IL5 promotes B cell growth and stimulates immunoglobulin secretion. It also induces eosinophil activation, proliferation, and differentiation.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Application |
Protein Quantification
|
---|---|
Brand |
HTRF
|
Detection Modality |
HTRF
|
Product Group |
Kit
|
Sample Volume |
16 µL
|
Shipping Conditions |
Shipped in Dry Ice
|
Target Class |
Cytokines
|
Target Species |
Human
|
Technology |
TR-FRET
|
Therapeutic Area |
Oncology & Inflammation
|
Unit Size |
500 Assay Points
|
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Revvity also worked with Myassays.com to help you in your data analysis.
Sample size | 16 µL |
---|---|
Final assay volume | 20 µL |
Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol |
LOD &LOQ (in Diluent) | 15 pg/mL &71 pg/mL |
Range | 71 20,000 pg/mL |
Time to result | 2h at RT |
Calibration | NIBSC (90/586) value (IU/mL) = 0,012 x HTRF hIL5 value (pg/mL) |
Species | Human only |
Intra-assay (n=24)
Sample | Mean [IL5] (pg/mL) | CV |
---|---|---|
1 | 104 | 9% |
2 | 969 | 2% |
3 | 8064 | 2% |
Mean CV | 4% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
Sample | [IL5] (pg/mL) | Mean (delta R) | CV |
---|---|---|---|
1 | 388 | 552 | 4% |
2 | 1878 | 2413 | 4% |
3 | 4131 | 5060 | 4% |
Mean CV | 4% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
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