Interleukin 22 is a type 2 cytokine (member of the IL10 family) and plays an essential role in innate protective immunity against extracellular pathogens. It induces pro-inflammatory responses, allows production of antimicrobial peptides. IL-22 is also involved in wound-healing and regeneration of epithelial cells. Human IL-22 is produced on inflammation site by Th17 CD4+ cells, activated T-cells, Th1 and NK cells. After being secreted, it targets non-hematopoietic cells and binds to IL22R that is restricted to tissue-resident cells (fibroblasts, epithelial cells). Downstream signaling involves phosphorylation of Jak1 and Tyk2 and subsequent activation of STAT3. Cisbio’s Human IL22 kit is a homogeneous, add and read assay to measure IL-22 secretion in cell supernatants. It offers a fast, efficient alternative to ELISA.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.