The HTRF human IL12 kit is designed for the quantification of IL12 release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Also known as NKSF (natural killer cell stimulatory factor) and CLMF (cytotoxic lymphocyte maturation factor), IL12 is produced by macrophages and B cells. Along with IFN gamma, IL12 promotes the differentiation of naïve T cells into TH1 cells, which in turn release IFNg and IL2. Because of its effects on TH1 induction, IL12 is strongly linked to autoimmunity. IL12 is a heterodimer of p35 and p4,0 which is shared with IL23. Revvity's IL12 assay is specific for IL12p70 (full form) and does not cross react with IL23.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma.
Assay Points |
500
|
---|---|
Assay Target Type |
Kit
|
Assay Technology |
HTRF
|
Brand |
HTRF
|
Quantity |
1
|
Shipping Conditions |
Shipped in Dry Ice
|
Therapeutic Area |
Oncology & Inflammation
|
Unit Size |
500 Assay Points
|
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Revvity also worked with Myassays.com to help you in your data analysis.
Sample size | 16 µL |
---|---|
Final assay volume | 20 µL |
Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
LOD &LOQ (in Diluent) | 14 pg/mL &81 pg/mL |
Range | 81 13,000 pg/mL |
Time to result | 2h at RT |
Calibration | NIBSC (95/544) value (IU/mL) = 0,01 x HTRF hIL12 value (pg/mL) |
Species | Human only |
Intra-assay (n=24)
Sample | Mean [IL12] (pg/mL) | CV |
---|---|---|
1 | 200 | 6% |
2 | 5000 | 4% |
3 | 10000 | 3% |
Mean CV | 4% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
Sample | [IL12] (pg/mL) | Mean (delta R) | CV |
---|---|---|---|
1 | 252 | 444 | 8% |
2 | 1221 | 2158 | 11% |
3 | 5909 | 10789 | 12% |
Mean CV | 10% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
This guide provides you an overview of HTRF applications in several therapeutic areas.
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