HTRF human IL1-β kit is designed quantification of human IL1-β release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
IL1 beta, also known as leukocytic pyrogen or mononuclear cell factor, is a pro-inflammatory cytokine of IL1 family. IL1 beta is considered to be a major endogenous pyrogen and induces prostaglandin synsis, T cell activation, B cell activation, and subsequent antibody production. It is also a known promoter of T cell differentiation into Th17.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Assay Points |
96
|
---|---|
Assay Target Type |
Kit
|
Assay Technology |
HTRF
|
Brand |
HTRF
|
Quantity |
1
|
Shipping Conditions |
Shipped in Dry Ice
|
Therapeutic Area |
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
|
Unit Size |
96 Assay Points
|
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page. Revvity also worked with Myassays.com to help you in your data analysis. you’ll be able to access a free online software to run your IL1 beta analysis.
Sample size | 16 µL |
---|---|
Final assay volume | 20 µL |
Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
LOD &LOQ (in Diluent) | 5 pg/mL &39.4 pg/mL |
Range | 39.4 6,500 pg/mL |
Time to result | Overnight at RT |
Calibration | NIBSC (86/680) value (IU/mL) = 0,1 x HTRF hIL1ß value (pg/mL) |
Species | Human only |
Intra-assay (n=24)
Sample | Mean [IL1ß] (pg/mL) | CV |
---|---|---|
1 | 65 | 18% |
2 | 705 | 9% |
3 | 5030 | 4% |
Mean CV | 10% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
Sample | [IL1ß] (pg/mL) | Mean (delta R) | CV |
---|---|---|---|
1 | 159 | 378 | 15% |
2 | 702 | 1742 | 13% |
3 | 3095 | 7736 | 12% |
Mean CV | 13% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
This guide provides you an overview of HTRF applications in several therapeutic areas.
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