US
Search Results
Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
HTRF Human IL-1β Detection Kit, 96 Assay Points
HTRF human IL1-β kit is designed quantification of human IL1-β release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
IL1 beta, also known as leukocytic pyrogen or mononuclear cell factor, is a pro-inflammatory cytokine of IL1 family. IL1 beta is considered to be a major endogenous pyrogen and induces prostaglandin synsis, T cell activation, B cell activation, and subsequent antibody production. It is also a known promoter of T cell differentiation into Th17.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Specifications
| Assay Points |
96
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Therapeutic Area |
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
|
| Unit Size |
96 Assay Points
|
Video gallery
Citations
How it works
Assay Principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page. Revvity also worked with Myassays.com to help you in your data analysis. you’ll be able to access a free online software to run your IL1 beta analysis.
Assay details
Technical specifications of human IL1 beta kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 5 pg/mL &39.4 pg/mL |
| Range | 39.4 6,500 pg/mL |
| Time to result | Overnight at RT |
| Calibration | NIBSC (86/680) value (IU/mL) = 0,1 x HTRF hIL1ß value (pg/mL) |
| Species | Human only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [IL1ß] (pg/mL) | CV |
|---|---|---|
| 1 | 65 | 18% |
| 2 | 705 | 9% |
| 3 | 5030 | 4% |
| Mean CV | 10% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [IL1ß] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 159 | 378 | 15% |
| 2 | 702 | 1742 | 13% |
| 3 | 3095 | 7736 | 12% |
| Mean CV | 13% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Resources
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
Flyer
No-wash cytokine assay solutions
Cytokines play a vital role in both innate and adaptive immunity and are known for their ability to exert diverse functions on...
Flyer
Reagent solutions for autoimmunity research.
Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies
Application Note
Simultaneous detection of drug efficacy and toxicity by combining HTRF, AlphaLISA, or AlphaLISA SureFire Ultra with ATPlite
This application note demonstrates how compound’s mechanism of action and potential cytotoxic effects can be deciphered thanks to...
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
How can we help you?
We are here to answer your questions.
