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HTRF Human IL-1α Detection Kit, 500 Assay Points
The HTRF human IL1 alpha kit is designed for the quantification of human IL1 alpha release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Also known as hematopoietin 1, IL1 alpha is a pro-inflammatory cytokine mainly produced by activated macrophages, and epilial and endolial cells. Its main role is maintenance and protection of skin's barrier function. In response to infection, IL1 alpha stimulates fibroblast proliferation, increases blood neutrophils, and activates lymphocyte proliferation.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Therapeutic Area |
Oncology & Inflammation
|
| Unit Size |
500 Assay Points
|
Video gallery
How it works
Assay Principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page. Revvity also worked with Myassays.com to help you in your data analysis. you’ll be able to access a free online software to run your IL1 alpha analysis.
Assay details
Technical specifications of human IL1 alpha kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 8 pg/mL &20.5 pg/mL |
| Range | 20.5 – 5,000 pg/mL |
| Time to result | 2h at RT |
| Calibration | NIBSC (86/632) value (IU/mL) = 10 x HTRF hIL1a value (pg/mL) |
| Species | Human only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [IL1a] (pg/mL) | CV |
|---|---|---|
| 1 | 50 | 11% |
| 2 | 2500 | 2% |
| 3 | 4500 | 4% |
| Mean CV | 6% |
Each of the 3 samples was measured 24 times, and % CV iwas calculated for each sample.
Inter-assay (n=4)
| Sample | [IL1a] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 78 | 246 | 6% |
| 2 | 411 | 1298 | 7% |
| 3 | 2174 | 5964 | 7% |
| Mean CV | 7% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Resources
Guide
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This guide provides you an overview of HTRF applications in several therapeutic areas.
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SDS, COAs, Manuals and more
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