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HTRF Human GM-CSF Detection Kit, 500 Assay Points
The HTRF human GM-CSF kit is designed for the quantification of human GM-CSF release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Also known as CSF2 (colony-stimulating factor 2), GM-CSF (granulocyte-macrophage colony-stimulating factor) is a hematopoietic growth factor and an immune modulator. Under immune stimuli, it is produced by T lymphocytes, macrophages, endolial cells, and fibroblasts. It acts on precursor cells in bone marrow to initiate ir proliferation and differentiation into mature granulocytes and macrophages. GM-CSF stimulates multipotent progenitor cells.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Therapeutic Area |
Oncology & Inflammation
|
| Unit Size |
500 Assay Points
|
Video gallery
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Revvity also worked with myassays.com to help you in your data analysis.
Assay details
Technical specifications of human GM-CSF kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers & protocol. |
| LOD & LOQ (in Diluent) | 27 pg/mL & 47 pg/mL |
| Range | 47 – 4,500 pg/mL |
| Time to result | Overnight at RT |
| Calibration | NIBSC (09/136) value (IU/mL) = 0,07 x HTRF hGCSF HTRF |
| Species | Human only |
Analytical performance
Intra and inter assay
Intra assay (n=24)
| Sample | Mean [GM-CSF] (pg/mL) | CV |
|---|---|---|
| 1 | 81 | 17% |
| 2 | 2125 | 4% |
| 3 | 6011 | 10% |
| Mean CV | 10% |
Inter assay (n=4)
| Sample | [GM-CSF] (pg/mL) | Mean (delta ratio) | |
|---|---|---|---|
| 1 | 169 | 370 | 6% |
| 2 | 774 | 1654 | 7% |
| 3 | 4135 | 5313 | 6% |
| Mean CV | 7% |
Assay validation
GM-CSF secretion by PBMC co-stimulated with ionomycin and PMA
PBMCs, plated at 100,000 cells/well in a 96 well plate, were stimulated for 24h with 1µg/ml of ionomycin and with various concentrations of PMA. 16µl of supernatant were transferred into a 384 well (small volume) white detection plate to be analyzed by the Human GM-CSF Assay.
SDS, COAs, Manuals and more
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