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HTRF Human Frataxin Detection Kit, 500 Assay Points

This HTRF kit is designed for the rapid detection of human Frataxin in cell lysates.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature	Specification
Application	Protein Quantification
Sample Volume	16 µL

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This HTRF kit is designed for the rapid detection of human Frataxin in cell lysates.

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Product Variants

Unit Size: 500 Assay Points

Part #:

63ADK039PEG

List Price

USD 1,039.00

Unit Size: 10,000 Assay Points

Part #:

63ADK039PEH

List Price

USD 11,750.00

Product information

Overview

Frataxin is a protein localized in the mitochondrion that acts either as an iron chaperone or an iron storage protein. Frataxin mRNA is predominantly expressed in tissues with a high metabolic rate such as the liver, kidneys, adipose tissue, and heart. Reduced expression of Frataxin is the cause of Friedreich's ataxia (FRDA), a lethal neurodegenerative disease. The human Frataxin kit enables the fast monitoring of Frataxin produced by cells.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	16 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Biomarkers
Target Species	Human
Technology	TR-FRET
Therapeutic Area	Neuroscience Rare Diseases
Unit Size	500 Assay Points

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How it works

Assay principle

Human Frataxin is measured using a sandwich immunoassay involving two specific Frataxin antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of Frataxin present in the sample.

Assay protocol

The human Frataxin assay features a two-plate assay protocol, where cells are plated, stimulated, and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of Human Frataxin. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.