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HTRF Human CXCL1 Detection Kit, 500 Assay Points

The HTRF human CXCL1 (GRO alpha) kit is designed for the quantification of human CXCL1 release in cell supernatant

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature	Specification
Application	Protein Quantification
Sample Volume	16 µL

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The HTRF human CXCL1 (GRO alpha) kit is designed for the quantification of human CXCL1 release in cell supernatant

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Product Variants

Unit Size: 500 Assay Points

Part #:

62HCXC1PEG

List Price

USD 1,103.00

Unit Size: 10,000 Assay Points

Part #:

62HCXC1PEH

List Price

USD 10,290.00

Product information

Overview

Also called GRO alpha, KC NAP-3 (neutrophil activating protein 3), or MGSA-alpha (melanoma growth stimulating activity alpha), CXCL1 is a member of CXC chemokine family. Mainly expressed by macrophages, CXCL1 attracts neutrophils and is involved in angiogenesis, inflammation, and tumorigenesis.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	16 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Cytokines
Target Species	Human
Technology	TR-FRET
Therapeutic Area	Oncology & Inflammation
Unit Size	500 Assay Points

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How it works

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

Revvity also worked with Myassays.com to help you in your data analysis.

Assay details

Technical specifications of human CXCL1 kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	13 pg/mL &47 pg/mL
Range	47 4,000 pg/mL
Time to result	2h at 4°C
Species	Human only