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HTRF Human CXCL1 (GROα) Detection Kit, 500 Assay Points
The HTRF human CXCL1 (GRO alpha) kit is designed for the quantification of human CXCL1 release in cell supernatant
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Also called GRO alpha, KC NAP-3 (neutrophil activating protein 3), or MGSA-alpha (melanoma growth stimulating activity alpha), CXCL1 is a member of CXC chemokine family. Mainly expressed by macrophages, CXCL1 attracts neutrophils and is involved in angiogenesis, inflammation, and tumorigenesis.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Therapeutic Area |
Oncology & Inflammation
|
| Unit Size |
500 Assay Points
|
Video gallery
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Revvity also worked with Myassays.com to help you in your data analysis.
Assay details
Technical specifications of human CXCL1 kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 13 pg/mL &47 pg/mL |
| Range | 47 4,000 pg/mL |
| Time to result | 2h at 4°C |
| Species | Human only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [CXCL1] (pg/mL) | CV |
|---|---|---|
| 1 | 150 | 8% |
| 2 | 1500 | 6% |
| 3 | 3000 | 9% |
| Mean CV | 8% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [CXCL1] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 212 | 350 | 13% |
| 2 | 686 | 934 | 2% |
| 3 | 2222 | 1904 | 1% |
| Mean CV | 5% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Resources
Guide
Cytokine assays: a guide to success with HTRF
The definitive guide to setting up a successful cytokine assay
Many therapeutic areas require an understanding of cytokine release...
Brochure
HTRF assays and reagents catalog
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
Literature - Publication Review
Neuroinflammation research solutions with HTRF assays
Discover the cutting edge of neuroinflammation assay research
This literature review illustrates the possibilities and versatility...
Flyer
No-wash cytokine assay solutions
Cytokines play a vital role in both innate and adaptive immunity and are known for their ability to exert diverse functions on...
SDS, COAs, Manuals and more
Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.
Safety data sheet
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Certificate of analysis
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Lot number08ALot dateMay 23, 2026
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Lot number05ALot dateMay 23, 2026
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Lot number06FLot dateMarch 29, 2026
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Lot number06ELot dateMarch 29, 2026
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Lot number06CLot dateMay 1, 2025
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Lot number06BLot dateMay 1, 2025
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IFUs, Manuals & more
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Resource typeManualLanguageEnglishCountry-
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