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HTRF Human CCL4 (MIP1β) Detection Kit, 10,000 Assay Points
The HTRF human CCL4 (MIP1 beta) kit is designed for the quantification of human CCL4 release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Also known as MIP-1beta (macrophage inflammatory protein 1 beta), CCL4 is a chemokine secreted by activated leukocytes, lymphocytes, and endolial and muscle cells in response to microbial infection, ischemia, or asthma.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Specifications
| Assay Points |
10000
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Therapeutic Area |
Oncology & Inflammation
|
| Unit Size |
10,000 Assay Points
|
Video gallery
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. Revvity also worked with Myassays.com to help you in your data analysis.
Assay details
Technical specifications of human CCL20 kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 7 pg/mL &20 pg/mL |
| Range | 7 pg/mL &20 pg/mL |
| Time to result | 1h at RT |
| Species | Human Only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [CCL4] (pg/mL) | CV |
|---|---|---|
| 1 | 36 | 9% |
| 2 | 2263 | 5% |
| 3 | 4280 | 5% |
| Mean CV | 6% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [CCL4] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 78 | 657 | 13% |
| 2 | 411 | 2979 | 16% |
| 3 | 2174 | 13278 | 15% |
| Mean CV | 15% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Resources
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
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