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HTRF Amyloid β1-40 Detection Kit, 500 Assay Points
The Amyloid ß1-40 kit is designed for the simple and rapid quantification of the peptide in cell-based formats.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Amyloid ß1-40 and ß1-42 peptides are involved in the appearance of Alzheimer's disease symptoms. The production of these two amyloid peptides is regulated by the activity of 2 enzymes, ß and γ secretase, which cleave the amyloid precursor protein. This kit is intended to measure amyloid ß1-40 peptides in cell supernatant or in whole cells. This assay is a fast alternative to ELISA, thanks to the ease-of-use of our Add and Read method.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped Ambient
|
| Therapeutic Area |
Neuroscience
|
| Unit Size |
500 Assay Points
|
Video gallery
Citations
How it works
Assay principle
The HTRF Amyloid ß1-40 assay is a sandwich immunoassay using two monoclonal antibodies that recognize different epitopes within the ß1-40 peptide. One antibody is labelled with Eu3+-Cryptate and the other with XL-665. The assay can be used with whole cells or cell supernatant.
Assay protocol
The Amyloid ß1-40 assay features a streamlined protocol with only two incubation steps:
- Cell stimulation by target compounds
- Amyloid ß1-40 detection using HTRF reagents
The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further speed up the assay procedure. This protocol requires a single 24-hour incubation at 4°C following cell stimulation.
Assay details
Key features
| Detection limit: | 13.64 pg/mL |
|---|---|
| Dynamic range: | 25 to 1600 pg/mL |
| Sample compatibility: | Cell-based or cell supernatants |
| Specificity: | No cross-reaction with Amyloid ß 1-42 and ß 1-43 |
Resources
Brochure
HTRF assays and reagents catalog
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.
Safety data sheet
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Certificate of analysis
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Lot number11FLot dateJune 6, 2025
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Lot number11GLot dateJune 6, 2025
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IFUs, Manuals & more
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Resource typeManualLanguageEnglishCountry-
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