The Amyloid ß1-40 kit is designed for the simple and rapid quantification of the peptide in cell-based formats.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Amyloid ß1-40 and ß1-42 peptides are involved in the appearance of Alzheimer's disease symptoms. The production of these two amyloid peptides is regulated by the activity of 2 enzymes, ß and γ secretase, which cleave the amyloid precursor protein. This kit is intended to measure amyloid ß1-40 peptides in cell supernatant or in whole cells. This assay is a fast alternative to ELISA, thanks to the ease-of-use of our Add and Read method.
Assay Points |
500
|
---|---|
Assay Target Type |
Kit
|
Assay Technology |
HTRF
|
Brand |
HTRF
|
Quantity |
1
|
Shipping Conditions |
Shipped Ambient
|
Therapeutic Area |
Neuroscience
|
Unit Size |
500 Assay Points
|
The HTRF Amyloid ß1-40 assay is a sandwich immunoassay using two monoclonal antibodies that recognize different epitopes within the ß1-40 peptide. One antibody is labelled with Eu3+-Cryptate and the other with XL-665. The assay can be used with whole cells or cell supernatant.
The Amyloid ß1-40 assay features a streamlined protocol with only two incubation steps:
- Cell stimulation by target compounds
- Amyloid ß1-40 detection using HTRF reagents
The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further speed up the assay procedure. This protocol requires a single 24-hour incubation at 4°C following cell stimulation.
Detection limit: | 13.64 pg/mL |
---|---|
Dynamic range: | 25 to 1600 pg/mL |
Sample compatibility: | Cell-based or cell supernatants |
Specificity: | No cross-reaction with Amyloid ß 1-42 and ß 1-43 |
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