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HTRF Human Serum Albumin Kit, 500 Assay Points
The HSA kit is designed for the rapid detection of HSA in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Human Serum Albumin (HSA) is a protein produced in the liver that represents the most abundant component in human blood plasma. Serum Albumin's main function is the regulation of the colloidal osmotic pressure of blood. The kit is intended for the specific and fast quantification of HSA in cell supernatants.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Therapeutic Area |
Metabolism/Diabetes
|
| Unit Size |
500 Assay Points
|
Video gallery
Citations
How it works
Assay principle
The HSA assay is a sandwich immunoassay. The assay uses two specific anti-human serum albumin antibodies, one labelled with Eu3+-Cryptate and the other with d2. The specific signal is proportional to the concentration of HSA present in the sample. This homogeneous assay requires only a single reagent addition (by premixing detecting reagents) and an ON incubation for the quantitative detection of CHO HCP.
Assay Protocol
The HSA assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires only a single ON incubation period at RT.
Assay validation
HSA detection on Primary Human Hepatocytes
Primary Human Hepatocyte supernatants from different single donors (A&B) and donor pool (C&D) were thawed and placed in culture. The supernatants were tested the first day of culture and 4 days after.
Resources
Application Note
Multi-parametric high-throughput analysis of hepatotoxicity using a 3D primary liver cell culture model
3D cell cultures offer a more physiologically relevant option, but there are currently only a few high throughput screening (HTS)...
SDS, COAs, Manuals and more
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