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HTRF Cynomolgus TNFα Detection Kit, 500 Assay Points
The HTRF cynomolgus TNF alpha kit is intended for the quantification of cynomolgus TNF alpha release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
TNF alpha, mainly produced by activated macrophages, is one of main cytokines in acute phase reaction. Depending on cellular target and biological context, TNF alpha can activate pro-apoptosis or pro-survival pathways, thus regulating tissue development through apoptosis control. Outside of oncology and inflammation, TNF alpha contributes to development of type II diabetes by increasing insulin resistance and modulating fatty acid metabolism.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Therapeutic Area |
Metabolism/Diabetes
NASH/Fibrosis
Oncology & Inflammation
|
| Unit Size |
500 Assay Points
|
Video gallery
Citations
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Assay details
Technical specifications of Cynomolgus TNF alpha
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers & protocol |
| LOD & LOQ (in Diluent) | 12.4 pg/mL & 27 pg/mL |
| Range | 27 – 3,000 pg/mL |
| Time to result | ON at RT |
| Species | Cynomolgus only |
Analytical performance
Inter and Intra assay
| Sample | Mean [TNFa] (pg/mL) | CV |
|---|---|---|
| 1 | 100 | 14% |
| 2 | 1500 | 2% |
| 3 | 2750 | 2% |
| Mean CV | 6% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
| Sample | [TNFa] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 159 | 665 | 3% |
| 2 | 514 | 2150 | 2% |
| 3 | 1667 | 6467 | 2% |
| Mean CV | 2% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Resources
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
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