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HTRF CD32b (FcgRIIb) Cellular Binding Kit, 1,000 Assay Points
The CD32b cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD32b receptor on live cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
The CD32b (FcgRIIb) cellular binding assay is a simple and robust test to monitor binding of the IgG Fc region to the human CD32b receptor in live cells. The CD32b receptor negatively modulates the Antibody-Dependent Cell-Phagocytosis (ADCP) pathway. This assay, based on our innovative Tag-lite technology, can easily be automated to create a high-throughput solution for predicting ADCP.
Specifications
| Brand |
HTRF
|
|---|---|
| Unit Size |
1,000 Assay Points
|
Video gallery
How it works
Assay principle
The CD32b receptor cellular binding assay is a competitive assay involving HEK293 cells expressing a specific CD32b variant conjugated to Lumi4-terbium Cryptate, and human IgG1 labelled with d2. The unlabeled antibody or Fc tagged drug competes with the d2 labelled IgG1 for binding to the receptor, resulting in a decrease of FRET signal.
Assay protocol
The CD32b receptor cellular binding assays (FcgIIb) present only 1 incubation step after reagents distribution. Lumi4-terbium Cryptate pre-labelled cells are dispensed, followed by the addition of IgG1 labelled with d2 and the sample. After 2h incubation at RT, the signal can be read.
Resources
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
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