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HTRF CD16a (FcgRIIIa, F158) Cellular Binding Kit, 500 Assay Points
HTRF CD16a (FcgRIIIa, F158) Cellular Binding Kit, 500 Assay Points
Shipping box for Revvity reagent kits
The CD16a cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD16a receptor on live cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 5 µL |
The CD16a cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD16a receptor on live cells.
Product Variants
Unit Size: 100 Assay Points
Part #:
6FR3APAG
List Price
USD 1,053.00
Unit Size: 500 Assay Points
Part #:
6FR3APAE
List Price
USD 3,865.00
Unit Size: 1,000 Assay Points
Part #:
6FR3APAF
List Price
USD 6,399.00
HTRF CD16a (FcgRIIIa, F158) Cellular Binding Kit, 500 Assay Points
Shipping box for Revvity reagent kits
HTRF CD16a (FcgRIIIa, F158) Cellular Binding Kit, 500 Assay Points
Product information
Overview
The CD16a (FcgRIIIa, F158) cellular binding assay is a simple and robust test to monitor binding of the IgG Fc region to the human CD16a receptor in live cells and to select the most potent antibody for use in Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC). The assay can also be used to assess the degree of antibody afucosylation. Two separate CD16a assays are available to specifically target the V158 or F158 variants. These assays, based on our innovative Tag-lite technology, can easily be automated to create a high-throughput solution for predicting ADCC.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
5 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Biologics
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Rare Diseases
|
| Unit Size |
500 Assay Points
|
Video gallery
HTRF CD16a (FcgRIIIa, F158) Cellular Binding Kit, 500 Assay Points
HTRF CD16a (FcgRIIIa, F158) Cellular Binding Kit, 500 Assay Points
How it works
Assay principle
The CD16a receptor cellular binding assay is a competitive assay involving HEK293 cells expressing a specific CD16a variant conjugated to Lumi4-terbium Cryptate and human IgG1 labelled with d2. The unlabeled antibody or Fc tagged drug competes with the d2 labelled IgG1 for binding to the receptor, resulting in a decrease of FRET signal.
Assay protocol
The CD16a receptor cellular binding assays (FcgIIIa V158 or F158) present only 1 incubation step after reagents distribution. Lumi4-terbium Cryptate pre-labelled cells are dispensed, followed by the addition of IgG1 labelled with d2 and the sample. After 2h incubation at RT, the signal can be read.
Assay validation
Specificity for IgG subclasses
Increasing concentrations of unlabeled IgGs were incubated in presence of a constant concentration of d2-labeled IgG1. The plate was then read on a PHERAstar microplate reader (BMG Labtech). As previously described, it was confirmed that the affinity of IgG1 for CD16a is better than that of IgG3 and considerably higher than IgG2 and IgG4 (Bruhns et al., Blood 2009).
A-fucosylation assessment
To evaluate the impact of IgG a-fucosylation on CD16a affinity, the same IgG with various degrees of fucosylation was tested following the previously described protocol. IgG affinity for CD16a and degree of a-fucosylation were very well correlated. These results confirm that a substantial gain of immunoglobulin affinity can be reached when the level of a-fucosylation is higher than 50%. The CD16a assay can therefore be used for the prediction of a-fucosylation degree and ADCC potential.
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