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HTRF cAMP Gs HiRange Detection Kit, 1,000 Assay Points
This HTRF kit detects changes of cAMP accumulation in response to Gs coupled GPCR activation or inhibition
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Based on HTRF technology, the kit contains all the reagents needed to quantify cAMP, ensuring that you benefit from a complete tool for your needs.
Revvity's HTRF cAMP kits are best suited for measuring cAMP modulations in response to Gs-activation, leveraging an optimized combination of robustness and sensitivity
Specifications
| Assay Points |
1000
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped Ambient
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
1,000 Assay Points
|
Video gallery
How it works
Assay principle
Revvity cAMP kits are based on a competitive format involving a specific antibody labeled with cryptate (donor) and cAMP coupled to d2 (acceptor). This enables the direct characterization of all types of compounds acting on Gs-coupled receptors in either adherent or suspension cells.
Assay protocol
The protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents.
Assay details
cAMP Gs hirange product specifications
If your experimental design uses membrane preparations, lipoparticles or purified receptors, or if your samples have large quantities of cAMP, the Gs HiRange kit will fit your needs!
The kit shows no ATP cross-reactivity up to 10mM, allowing the user to operate with large ranges of ATP and enabling membrane work.
Designed for optimal signal performance, cAMP - Gs HiRange offers a maximized signal-to-background score.
| S/B | IC20 nM | IC50 nM | IC80 nM | |
|---|---|---|---|---|
| cAMP - Gs dynamic | >14 | 0.91 | 4.07 | 18.1 |
| cAMP - Gs HiRange | >28 | 4.27 | 22.8 | 121 |
ATP cross-reactivity
Resources
Guide
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SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
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