This Angiotensin receptor red agonist is an angiotensine II derivative labeled with a red emitting HTRF fluorescent probe.
For research use only. Not for use in diagnostic procedures.
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.
This Angiotensin receptor red agonist is an angiotensine II derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Angiotensin AT2 receptor binding assay.
Assay Points |
5000
|
---|---|
Assay Target Type |
Fluorescent reagent
|
Assay Technology |
HTRF
|
Brand |
Tag-Lite
|
Quantity |
1
|
Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
Unit Size |
5,000 Assay Points
|
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
This guide provides you an overview of HTRF applications in several therapeutic areas.
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