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HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
HTRF Angiotensin AT2R receptors red ago vial
This Angiotensin receptor red agonist is an angiotensine II derivative labeled with a red emitting HTRF fluorescent probe.
For research use only. Not for use in diagnostic procedures.
| Feature | Specification |
|---|---|
| Application | Receptor-Ligand Binding |
This Angiotensin receptor red agonist is an angiotensine II derivative labeled with a red emitting HTRF fluorescent probe.
For research use only. Not for use in diagnostic procedures.
Product Variant
Unit Size: 5,000 Assay Points
Part #:
L0007RED
List Price
USD 2,254.00
HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
HTRF Angiotensin AT2R receptors red ago vial
HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
Product information
Overview
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.
This Angiotensin receptor red agonist is an angiotensine II derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Angiotensin AT2 receptor binding assay.
Specifications
| Application |
Receptor-Ligand Binding
|
|---|---|
| Brand |
Tag-lite
|
| Detection Modality |
HTRF
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
GPCR
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
5,000 Assay Points
|
Video gallery
HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
How it works
Assay principle
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Resources
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Flyer
Tag-lite: the binding receptor assay portfolio addressing the adenosine receptor family
Tag-lite™ is a cell-based, no-wash alternative to traditional radioisotope assays for studying GPCR-ligand interaction.
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