Revvity Sites Globally

Select your location.

*e-commerce not available for this region.

Australia

Austria

Belgium

Brazil *

Canada

China *

Denmark

Finland

France

Germany

Hong Kong *

India *

Ireland

Italy

Japan *

Luxembourg

Mexico *

Netherlands

Norway

Philippines *

Republic of Korea *

Singapore *

Spain

Sweden

Switzerland

Thailand *

United Kingdom

United States

HTRF α-Synuclein Aggregation Kit, 500 Assay Points

This HTRF kit is designed for an accurate quantitative measurement of alpha synuclein aggregation in brain extracts.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

View product information

Feature	Specification
Application	Protein Quantification
Sample Volume	10 µL

Request More Information

This HTRF kit is designed for an accurate quantitative measurement of alpha synuclein aggregation in brain extracts.

View product information

Product Variants

Unit Size: 500 Assay Points

Part #:

6FASYPEG

List Price

USD 1,603.00

Unit Size: 10,000 Assay Points

Part #:

6FASYPEH

List Price

USD 18,890.00

Product information

Overview

Monitoring alpha-synuclein aggregation is of great interest for studying the pathogenesis of synucleinopathies, a group of neurodegenerative diseases that includes Parkinson's disease (PD), dementia with Lewy bodies (DLB), diffuse Lewy body disease (DLBD), and multiple system atrophy (MSA). The kit provides a solution to rapidly detect and quantify protein aggregates of human alpha-synuclein in cell culture or brain tissue extracts.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Biomarkers
Technology	TR-FRET
Therapeutic Area	Neuroscience
Unit Size	500 Assay Points

Video gallery

Citations

View product citations for 6FASYPEG on CiteAb

How it works

Assay principle

Alpha-Synuclein aggregates are measured using a sandwich immunoassay involving an anti-a Synuclein monoclonal antibody specific for alpha-Synuclein aggregate labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the alpha-Synuclein aggregates.

Assay Protocol

The sample or brain extracts are transferred to the assay plate for the detection of a-Synuclein aggregates. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Assay validation

Alpha synuclein aggregation in human brain samples

Samples from human putamen (Parkinson's versus control) were collected and lysed with a Revvity lysis buffer. Due to the nature of the samples, the conventional cellular protocol needed to be adjusted. Incubation was performed for 20 hours at RT. Even at high dilution (1/64), clear discrimination between the two sample types is possible with Revvity's aggregated alpha synuclein assay.