DELFIA® Europium-labeled streptavidin, for use in DELFIA time-resolved fluorescence (TRF) assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
DELFI® Eu-labeled streptavidin is intended for use as a generic reagent to detect biotin-labeled compounds, e.g. antibodies or antigens in various types of immunoassay or nucleic acid probes in hybridization assays based on solid-phase separation. The label, Eu3+, is measured using dissociation-enhanced time-resolved fluorometry.
The streptavidin is isolated from fermentation filtrates of Streptomyces avidinii and 1 mg of streptavidin binds approximately 10 μg of biotin. The preparation is supplied ready to use in 50 mmol/L Tris-HCl buffered saline solution. For DELFIA immunoassays, a final concentration of 100 ng/mL is recommended for the generation of a suitable signal.
Antibody Conjugates |
Streptavidin
|
---|---|
Application |
Protein-protein interactions
Protein Analysis & Detection
|
Automation Compatible |
Yes
|
Brand |
DELFIA
|
Detection Method |
DELFIA TRF
Time-Resolved Fluorescence (TRF)
|
Experimental Type |
In vitro
|
Fluorophore |
Eu-N1
|
Format |
Microplates
|
One Unit Contains |
250.0 µg
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
250 µg
|
Investigate the dynamics of Interactions
Kinetic values are of importance for characterizing protein-protein interactions...
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