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DELFIA Eu-N1 Streptavidin

DELFIA® Europium-labeled streptavidin, for use in DELFIA time-resolved fluorescence (TRF) assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part number: 1244-360
List price: USD 540.24
Your price:
USD 540.24
USD 540.24 /each

Overview

DELFI® Eu-labeled streptavidin is intended for use as a generic reagent to detect biotin-labeled compounds, e.g. antibodies or antigens in various types of immunoassay or nucleic acid probes in hybridization assays based on solid-phase separation. The label, Eu3+, is measured using dissociation-enhanced time-resolved fluorometry.

The streptavidin is isolated from fermentation filtrates of Streptomyces avidinii and 1 mg of streptavidin binds approximately 10 μg of biotin. The preparation is supplied ready to use in 50 mmol/L Tris-HCl buffered saline solution. For DELFIA immunoassays, a final concentration of 100 ng/mL is recommended for the generation of a suitable signal.

Specifications

Antibody Conjugates
Streptavidin
Application
Protein-protein interactions
Protein Analysis & Detection
Automation Compatible
Yes
Brand
DELFIA
Detection Method
DELFIA TRF
Time-Resolved Fluorescence (TRF)
Experimental Type
In vitro
Fluorophore
Eu-N1
Format
Microplates
One Unit Contains
250.0 µg
Shipping Conditions
Shipped in Blue Ice
Unit Size
250 µg

Citations

Resources

1-6 of 6 Resources
Application Note Icon
Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

Guide Icon
Guide
DELFIA immunoassay development guide

DELFIA immunoassays are a superior alternative to traditional ELISAs

Technical Note Icon
Technical Note
How to access true Kd values in Protein-Protein Interaction assays

Investigate the dynamics of Interactions

Kinetic values are of importance for characterizing protein-protein interactions...

Application Note Icon
Application Note
Optimization and miniaturization of DELFIA TRF assays converted from ELISA.

This detection method is similar to a traditional ELISA, however DELFIA’s utilization of lanthanide metals endows DELFIA with...

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Guide
The ultimate guide to PPI assays with HTRF

Your dream assay does not exist yet? Create it!

This guide will provide you with tools to help you develop your very own protein...

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Guide
Time-resolved fluorometry with the DELFIA method user guide

This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

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