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Cellometer X2 Fluorescent Cell Viability Counter

The Cellometer™ X2 automated cell counter is optimized for brewing yeast, wine yeast, platelets and other small cells.

For research use only. Not for use in diagnostic procedures.

Product Variant
Unit Size: 1 Each
Part #:
CMT-X2-S150

Overview

Our yeast automated cell counter, the Cellometer X2, can be up to five times faster than manual cell counting. The cell count, concentration, diameter, and % viability are automatically calculated and reported within less than 60 seconds.

The Cellometer X2 is optimized for live yeast cell concentration and viability counting. It can also be used for:

  • Platelets
  • Other small cells

Additional product information

Fluorescent analysis

Fluorescent dyes that stain DNA can be used to identify nucleated cells in a mixed cell population. Acridine orange (AO) is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence when imaged with the Cellometer X2.

Because mature mammalian red blood cells don’t contain nuclei, only mononuclear cells produce a fluorescent signal. Fluorescent-positive nucleated cells are easily counted. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step.

x4 nucleated cell counting
With propidium iodide (PI), dead cells are clearly visible in the red fluorescence channel

Use of a fluorescent dye, such as propidium iodide (PI), is highly recommended for accurate viability analysis of cell samples containing debris.

Propidium iodide (PI) is a nuclear staining (nucleic acid binding) dye that enters dead cells with compromised membranes. It stains all dead nucleated cells to generate red fluorescence.

Because the Cellometer X2 can count dead cells in the red fluorescence channel, there is no interference from debris and live cells.

fluorescent viability determination
1-Step yeast concentration & viability analysis using Cellometer X2

Brightfield and fluorescent imaging

The Cellometer X2 Image Cytometer performs simple, 1-step determination of yeast concentration and viability. The Cellometer X2 is useful for research laboratories looking to automate fermentation monitoring.

Brightfield images can be viewed to confirm cell morphology. Counted brightfield images show counted cells within cell clumps. Fluorescent images show dead cells for confirmation of viability results. Analysis of both brightfield and fluorescent images from each sample makes 1-step concentration & viability determination possible.

1-step yeast concentration viability

Brightfield and fluorescent images of yeast cells. Cells appearing in both the brightfield and fluorescent image are dead.

Exclusion of debris & size-based cell counting

Because Cellometer recognizes cells based on size, brightness, and morphology, cellular debris is easily and accurately excluded from counting results.

  • Cell size parameters can be modified to optimize exclusion of debris from results and enhance the accuracy of counting for a wide range of cell sizes.
  • A fluorescent nuclear dye, such as acridine orange, can be used to more easily identify nucleated cells in samples containing debris.
cell size histogram

The Cellometer X2 Image Cytometer automatically generates a cell size histogram based on cell diameter.

cell count optimized to exclude debris

The minimum and maximum cell diameter settings can be optimized to count specific cells in a sample.

Dynamic range of total cells with Cellometer X2

Concentration dynamic range figure 1 depicts the dynamic range for cell concentration measured by Cellometer X2. This data set was taken on a concentration series of cultured yeasts.

Samples from 2.5 x 105 - 5 x 107 cells/ml can be counted without further dilution.

The %CV at each concentration was below 10%.

dynamic range of total cells with X2

Figure 1: Table of results for cell concentration dynamic range.

Cellometer X2 repeatability and consistency

The results provide evidence of the increased accuracy of the Cellometer X2 instrument in assessing the viability of yeasts using AOPI for cell viability. Yeasts were tested at 24 sample replications. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer X2 in measuring cell concentration and viability of yeast cells.

Cellometer X2 Average live cell concentration via fluorescence Viability
AVE 1.32E+07 78.1%
STDEV 7.69E+05 2.2%
%CV 5.84 2.78


Table of results for cell concentration and viability using acridine orange (AO) and propidium Iodide (PI).
 

Cellometer X2 specifications
Includes
  • Cellometer X2 instrument
  • Cellometer software
  • Two standard fluorescence optics module
  • Power supply
  • USB 2.0 connection cable
  • Phone/online applications support during set-up
Available accessories
  • PC laptop
Imaging performance
  • Cell Size: 0.5 - 15 microns
  • Concentration Range: 5 x105 – 107 cells/ml
  • Brightfield imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.
Instrument specifications
  • Weight: 23.0 lbs. (10.4 kg)
  • Dimensions: Width: 6.0” (15.2 cm), Depth: 8.5” (21.6 cm), Height: 14.0” (35.6 cm)
  • Input to power adapter: 100-240 VAC, 50/60 Hz, 1.0A
  • Output to instrument: 12 VDC, 3.34A

PC / Laptop minimum requirements:

(If purchasing Cellometer without PC laptop)

  • Windows 10
  • Intel® i5 (1.6 - 4.40 GHz) Processor
  • 10 Core
  • 4 GB RAM
  • Integrated Graphics Card
  • 1080p Display Resolution
  • 500 GB Hard Drive
  • USB 2.0 Port

 

Available fluorescence optics modules

VC-535-403

Excitation / Emission: 470nm/535nm

Example fluorophores:

  • Acridine Orange
  • CFDA
  • FITC

VC-660-503

Excitation / Emission: 540nm/660nm

Example fluorophores:

  • Propidium iodide (PI)
  • Ethidium bromide
  • 7-AAD

Specifications

Brand
Cellometer
Unit Size
1 Each

Resources

Are you looking for resources, click on the resource type to explore further.

1-3 of 3 Resources
Application Note Icon
Application Note
A quick and direct Candida albicans lysis efficiency comparison using the Cellometer X2 fluorescent cell viability counter.

Application note for Cellometer X2Candida albicans lysis efficiency

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Application Note
Reliable quantification of Candida albicans by Cellometer X2 cell counter: correlation study with qPCR and ddPCR

Application note for Cellometer X2 correlation study with qPCR and ddPCR

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Application Note
Yeast concentration and viability using image-based fluorescence analysis using the Cellometer X2 fluorescent cell viability counter.
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