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Cellometer Spectrum Image Cytometry System

The Cellometer™ Spectrum image cytometer is an advanced, modular cell counter capable of running cell-based assays utilizing fluorescent reagents and kits. It can be customized for specific assays/reagents of interest.

For research use only. Not for use in diagnostic procedures.

Product Variant
Unit Size: 1 Each
Part #:
SPECTRUM-SYS1-10X

Overview

The Cellometer Spectrum image cytometer is a customizable cell counting and cytometry instrument that provides flow-like data with predesigned templates.

You can quickly plot cell population data as a histogram, scatter plot, dot plot or contour plot using FCS express. The filter sets are user-changeable, allowing you to customize the instrument for specific assays/reagents of interest.

Integrated cell analysis solution

Basic cell counting, primary cell viability, and cell-based assays.

  • PBMCs​
  • Stem Cells​
  • Adipocytes​
  • Neural Cells​
  • Hepatocytes​
  • Dendritic Cells​
  • Epithelial Cells​
  • Keratinocytes​
  • Lymphocytes​
  • Splenocytes​
  • Monocytes​
  • Yeast​
  • Leukocytes​
  • Cell Lines​
  • Nucleated Cells

Dual-fluorescence for accurate primary cell viability

No interference from red blood cells. Analyze bone marrow, peripheral blood, and cord blood without lysing.

User changeable fluorescence filters

Choose from six color options to run two-color assays

Unique algorithms for advanced cell analysis

Determine concentration and viability of hepatocytes, adipocytes, and other sophisticated cell types.

Fast results

Obtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.

Simple cell-based assays

  • Small 20 µl sample size
  • Cell count, concentration and viability
  • Two-color antibody assays
  • GFP/RFP Transfection
  • Cell health and cell-based assays, including:
    • Apoptosis
    • Cell proliferation
    • Cell cycle
    • Mitochondrial potential
    • Phagocytosis
    • Surface Marker Analysis

Additional product information

Advantages of Cellometer Spectrum

View Cell Images

  • Visually check cell morphology
  • Ensure only cells of interest are counted
  • Archive and re-analyze cell images
  • Export images for publication
     

Proprietary Pattern-Recognition Software

  • Count individual cells in clusters
  • Count irregular-shaped cells
  • Count cells based on size
  • Eliminate debris from cell counts
     

Non-Fluidic Platform

  • Disposable counting chambers – no washing
  • Compatible with fragile cells
  • Maintenance-free
  • Robust optics modules and LED light sources
     

IQ/OQ Validation and GMP/GLP Accessories

  • Installation Qualification reagents/protocol
  • Operational Qualification reagents/protocol
  • On-site IQ and OQ performance
  • GMP/GLP Software Module


Fluorescent Cell Counting

Brightfield and AO cell image
  • Automatic identification of Acridine Orange (AO) stained cells (shown above) and Propidium Iodine (PI) stained cells (shown below).
  • Image and count up to 2×107cells/mL
Brightfield and PI cell image


User-Changeable Fluorescence Optics Modules*

The Spectrum is designed to hold two user-changeable fluorescence optics modules. Purchase only the modules you need and easily configure the instrument by quickly changing the colors to fit your experimental design.

Optics Module Fluorophores Nucleic Acid Stains Fluorescent Proteins and other Fluorescent Cell Reagents
S1-452-365

Ex. 370 nm (BW: 36 nm)

Em: 452 nm (BW: 45 nm)
BV421

V450

Pacific Blue
Hoechst 33342

DAPI

ViaStain Dead Cell Nuclear Blue
Calcein AM Violet

CTV (CellTrace Violet)

Tracer Blue

BFP
S1-534-470

Ex: 475 nm (BW: 42 nm)

Em: 534 nm (BW:42 nm)
FITC

AlexaFluor® 488
AO (acridine orange)

SYTO®9, SYTO®13

SYTOX®Green

SYTO®BC
GFP

YFP

Calcein AM

CFSE

JC-1
S1-594-470

Ex. 475 nm (BW: 42 nm)

Em: 594 nm (LP - Long Pass)
    Chlorophyll A

Chlorophyll B
S1-605-527

Ex: 525 nm (BW: 45 nm)

Em: 605 nm (BW: 64 nm)
AlexaFluor® 546

AlexaFluor® 555

Cy3®

PE (R-phycoerythrin)

RFP
PI (propidium iodide)

EB (ethidium bromide)

SYTOX® Orange
RFP

mCherry

TdTomato

TurboRed

TMRE/TMRM

JC-1
S1-655-527

Ex: 525 nm (BW: 45 nm)

Em: 655 nm (BW: 40 nm)
  PI (propidium iodide)

EB (ethidium bromide)

7-AAD
Nile Red
S1-692-620

Ex: 628 nm (BW: 40 nm)

Em: 692 nm (BW: 40 nm)
AlexaFluor® 647

APC (allophycocyanin)

Cy5®
SYTOX® Red iRFP670

CellTrace Far Red

Cell Tracker Deep Red

LysoTracker Deep Red

 

*This table is a partial list of compatible fluorophores, nucleic acid stains, and fluorescent proteins. Please contact Revvity technical support regarding compatibility of other reagents. Sytox, AlexaFluor, and Cy are trademarks of Life Technologies.
 

Cellometer Spectrum Specifications 

Includes
  • Cellometer Spectrum image cytometer
  • Cellometer Software
  • FCS Express 6 analysis software (2 licenses)
  • Two Fluorescent Optics Modules
  • Power Supply
  • USB 2.0 Connection Cable
  • Phone/online applications support during set-up
Magnification selection
  • 5x (ex. most mammalian cells)
  • 10x (ex. yeast, platelets, algae)
Instrument specifications
  • Weight: 24.0 lbs. (10.9 kg)
  • Dimensions: Width: 6.0" (15.2 cm), Depth: 8.5" (21.6 cm), Height: 14.0" (35.6cm)
  • Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.0A
  • Output to Instrument: 12 VDC, 3.34A
Available accessories
  • PC / Laptop
  • Minimum Requirements (If purchasing Cellometer without PC laptop)
Imaging performance
  • 5x magnifcation:
    • Cell Size: 5 - 300* microns
    • Concentration Range: 105 - 107 cells/ml
  • 10x Magnification:
    • Cell Size: 0.5 - 25 microns
    • Concentration Range: 105 - 107 cells/ml
  • Brightfield imaging, fluorescent imaging and pattern-recognition software to quickly decluster, identify and count individual cells.

* Cellometer CHT4-PD300 slides are required for cells > 80 microns in diameter

Specifications

Brand
Cellometer
Unit Size
1 Each

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