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HTRF β-arrestin 2 recruitment Detection Kit, Control Lysate
HTRF β-arrestin 2 recruitment Detection Kit, Control Lysate
Picture of HTRF β-Arrestin 2 Control Lysate
This lysate can be used with the B-arr2 recruitment kit. It may be used as a positive control for B-arrestin 2 recruitment of AP2.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Second Messenger Detection |
This lysate can be used with the B-arr2 recruitment kit. It may be used as a positive control for B-arrestin 2 recruitment of AP2.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Product Variant
Unit Size: 1 Vial
Part #:
62BDBAR2TDA
List Price
USD 453.17
HTRF β-arrestin 2 recruitment Detection Kit, Control Lysate
Picture of HTRF β-Arrestin 2 Control Lysate
HTRF β-arrestin 2 recruitment Detection Kit, Control Lysate
Product information
Overview
This cell lysate provides a way to validate the B-arr2 recruitment assay. As a positive control, it will enable researchers to avoid any detection issues when running the assay. The lysate was made from transfected HEK293 cells.
Specifications
| Application |
Second Messenger Detection
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
GPCR
|
| Technology |
TR-FRET
|
| Unit Size |
1 Vial
|
How it works
Principle of the HTRF Beta-Arrestin2 Recruitment assay
The positive control is used as an internal assay control to check the quality of the results obtained with the pre-mixed d2 and Eu cryptate antibodies used for the Beta-arrestin2 recruitment assay.
Assay validation
Signal to background of beta-arrestin2 recruitment control lysate
5mL of Control Lysate were dispensed into all wells of a 96-well plate, followed by 100mL of pre-mixed d2 and Cryptate-labelled antibodies from the HTRF Beta-Arrestin 2 Recruitment Kit (62BDBAR2PEB/ (62BDBAR2PEC), as described in the corresponding protocol.
The plate was sealed and incubated overnight at room temperature, then read with an HTRF-compatible reader with a flash lamp.
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Technical Note
A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay
G-protein coupled receptors (GPCRs) are crucial transmembrane proteins involved in cellular signal transduction.
This technical...
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