Sequestome-1 or ubiquitin binding protein p62 (SQSTM1 p62) is an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. SQSTM1 p62 is an adaptor protein which is initially phosphorylated by ULK1 on Serine 407, then on Serine 403 by casein kinase 2 or TBK1. The phosphorylation on Ser403 increases its affinity for ubiquitin chains, thus enabling SQSTM1 p62 to bind to ubiquitinated cargo proteins. Ubiquitinated proteins or ubiquitin coated mitochondria associated with SQSTM1 p62 proteins are taken away in phagosomes, whose content is cleared out after lysosomal fusion. SQSTM1 p62 is implicated in Alzheimer’s disease, Huntington’s disease, NASH, and numerous types of cancer.

The AlphaLISA SureFire Ultra Human and Mouse Total SQSTM1 p62 Detection Kit is a sandwich immunoassay for the quantitative detection of total SQSTM1 p62 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening