Cyclin dependent kinase 9 (CDK9) is a member of the CDK family. CDK9 was found to be a component of the multiprotein complex TAK/P-TEFb which regulates transcription elongation through phosphorylation of the C-terminal tail domain (CTD) of RNA polymerase II (RNAP II). This crucial function combined with evidence that transcriptional programs are dysregulated in cancer make CDK9 an attractive target for cancer therapies.

The AlphaLISA SureFire Human and Mouse Total CDK9 Detection Kit is a sandwich immunoassay for the quantitative detection of total CDK9 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening