The AlphaLISA™ SureFire® Ultra™ phospho-AKT1 (Ser473) assay kit can be used to measure levels of AKT1 phosphorylated at residue Ser473 in cellular lysates using no-wash Alpha technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
In the AlphaLISA™ SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA™ SureFire® Ultra™ kits are compatible with:
Alpha SureFire® kits can be used for:
Application |
Kinase
Protein Analysis & Detection
|
---|---|
Assay Pathway |
PI3K/Akt/mTOR
|
Assay Points |
500
|
Assay Target |
Akt1
|
Assay Target Class |
Phospho-protein
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Molecular Modification |
Phosphorylation
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Cardiovascular
Central Nervous System
Metabolic
|
Unit Size |
500 Assay Points
|
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
An in-depth review of molecular and cellular pathways
The maintenance of proteostasis, the biological mechanisms that control the...
Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.
We are here to answer your questions.