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AlphaLISA SureFire Ultra Human & Mouse Phospho-AKT1/2/3 (Thr450) Detection Kit, 500 Assay Points

The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-AKT 1/2/3 (Thr450) assay is a sandwich immunoassay for quantitative detection of phospho-AKT 1/2/3 (Thr450) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Cell Signaling
Sample Volume 10 µL

The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-AKT 1/2/3 (Thr450) assay is a sandwich immunoassay for quantitative detection of phospho-AKT 1/2/3 (Thr450) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
ALSU-PAKT-C500
List Price
USD 2,486.00
Unit Size: 10,000 Assay Points
Part #:
ALSU-PAKT-C10K
List Price
USD 15,110.00
Unit Size: 50,000 Assay Points
Part #:
ALSU-PAKT-C50K
List Price
USD 48,780.00
Unit Size: 100 Assay Points
Part #:
ALSU-PAKT-C-HV
List Price
USD 740.59

Overview

Protein kinase B (PKB) also known as AKT1 AKT2 and AKT3 encode the RAC alpha, beta, and gamma serine/threonine protein kinases respectively. AKT1 is involved in cellular survival pathways, by inhibiting apoptotic process. It is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy and general tissue growth. Akt2 is an important signaling molecule in the insulin signaling pathway. AKT3 appears to be predominantly expressed in the brain.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-AKT 1/2/3 (Thr450) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-AKT 1/2/3 (Thr450) in cellular lysates, using Alpha Technology.

Formats:
  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with:
  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies
Alpha SureFire kits can be used for:
  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
AKT 1/2/3
Target Class
Phosphoproteins
Target Species
Human
Mouse
Technology
Alpha
Therapeutic Area
Cardiovascular
Oncology
Unit Size
500 Assay Points

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