The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-AKT 1/2/3 (Thr450) assay is a sandwich immunoassay for quantitative detection of phospho-AKT 1/2/3 (Thr450) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Cell Signaling |
Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-AKT 1/2/3 (Thr450) assay is a sandwich immunoassay for quantitative detection of phospho-AKT 1/2/3 (Thr450) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Protein kinase B (PKB) also known as AKT1 AKT2 and AKT3 encode the RAC alpha, beta, and gamma serine/threonine protein kinases respectively. AKT1 is involved in cellular survival pathways, by inhibiting apoptotic process. It is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy and general tissue growth. Akt2 is an important signaling molecule in the insulin signaling pathway. AKT3 appears to be predominantly expressed in the brain.
The AlphaLISA SureFire Ultra Human and Mouse Phospho-AKT 1/2/3 (Thr450) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-AKT 1/2/3 (Thr450) in cellular lysates, using Alpha Technology.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Kit
|
Sample Volume |
10 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
AKT 1/2/3
|
Target Class |
Phosphoproteins
|
Target Species |
Human
Mouse
|
Technology |
Alpha
|
Therapeutic Area |
Cardiovascular
Oncology
|
Unit Size |
500 Assay Points
|
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