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HTRF HIV p24 Detection Kit, 10,000 Assay Points

The HTRF HIV p24 Detection kit is designed for the detection and quantification of HIV p24 in buffered solution or cell culture medium.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Protein Quantification
Dynamic Range 33 - 10,000 pg/mL
Limit of Detection 12 pg/mL
Sample Volume 16 µL

The HTRF HIV p24 Detection kit is designed for the detection and quantification of HIV p24 in buffered solution or cell culture medium.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Unit Size: 500 Assay Points
Part #:
64P24PEG
List Price
USD 1,372.00
Unit Size: 10,000 Assay Points
Part #:
64P24PEH
List Price
USD 14,590.00

Overview

Immunodeficiency Virus (HIV) p24 is the 231 amino acid phosphorylated protein of the capsid forming the conical core of the virus, which encapsulates the genomic RNA-nucleocapsid complex. p24 is a cleavage product of the p55 Gag polyprotein by viral proteases. HIV p24 and its 55 kDa precursor play crucial roles in the assembly, maturation, and disassembly of HIV. e therapy, HIV-derived lentiviral vectors are useful and commonly implemented to deliver transgenes to mammalian cells. Assays against the p24 antigen have become a standard method for measuring lentiviral transduction efficiency.

Specifications

Application
Protein Quantification
Brand
HTRF
Detection Modality
HTRF
Dynamic Range
33 - 10,000 pg/mL
Limit of Detection
12 pg/mL
Product Group
Kit
Sample Volume
16 µL
Shipping Conditions
Shipped in Dry Ice
Target Class
Biologics
Technology
TR-FRET
Therapeutic Area
Infectious Diseases
Unit Size
10,000 Assay Points

Video gallery

Assay details

Key features

16 µL of recombinant protein in diluent #5 were dispensed into a low volume white microplate before the addition of 4 µL of the premixed HTRF anti HIVp24 detection reagents. The HTRF signal was recorded after 2h incubation at RT.

Sample size 16 µL
Final assay volume 20 µL
Time to results 2h at RT
Detection limit 12 pg/mL
Dynamic range 33 to 10,000 pg/mL
Sample compatibility Cell supernatant

 

specifications-hiv-p24

 

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HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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