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PhenoVue 577 Lysosomal stain
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PhenoVue 577 Lysosomal stain
PhenoVue 577 Lysosomal stain is a fluorescent dye which accumulates in acidic vesicles such as the lysosomes. The bright red fluorescence associated with PhenoVue 577 Lysosomal stain are validated for use in imaging microscopy and High Content Screening Systems.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
| Feature | Specification |
|---|---|
| Color | Red/Orange |
| Filter | RFP |
| Organelle and Cell Compartment | Lysosomes |
PhenoVue 577 Lysosomal stain is a fluorescent dye which accumulates in acidic vesicles such as the lysosomes. The bright red fluorescence associated with PhenoVue 577 Lysosomal stain are validated for use in imaging microscopy and High Content Screening Systems.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
PhenoVue 577 Lysosomal stain
PhenoVue 577 Lysosomal stain
Product information
Overview
Lysosomes are abundant organelles which contain more than sixty acidic enzymes. Lysosomes play essential role in macromolecules and organelles break down through the autophagy-lysosomal system. Defective regulation of lysosomal function has been reported in several diseases such as lysosomal storage disorders and neurodegenerative diseases like Alzheimer’s and Parkinson’s. PhenoVue 577 lysosomal stain is partially protonated at neutral pH, and highly protonated at acidic pH. In living cells, PhenoVue 577 lysosomal stain accumulates in acidic vesicles such as lysosomes, resulting in bright and specific lysosomal fluorescent staining. PhenoVue 577 lysosomal stain can be used to lysosomes in immunofluorescence, as well as high-content analysis and screening applications.
Additional product information
Features
| Numbers of vials per unit | 20 |
|---|---|
| Quantity or volume per unit | 19.9µg (50nmoles) |
| Form | Solution 1mM DMSO |
| Storage | -16°C or below |
| Recommended working concentration | 50 ng/mL |
| Maximum excitation wavelength | 577 nm |
| Maximum emission wavelength | 590 nm |
| Common filter set | RFP |
| Live cell staining | Yes |
| Fixed cell staining | No |
| Equivalent number of microplates | 650 - 2050 x 96-well microplates 550 - 2050 x 384-well microplates 1050 - 3250 x 1536-well microplates |
Specifications
| Color |
Red/Orange
|
|---|---|
| Form |
1mM solution in DMSO
|
| Maximum Emission Wavelength (Emmax) |
590 nm
|
| Maximum Excitation Wavelength (Exmax) |
577 nm
|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Filter |
RFP
|
| Organelle and Cell Compartment |
Lysosomes
|
| Quantity |
20 x 50 µL
|
| Sample Type |
Live samples only
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Storage Conditions |
-16 °C or below, protected from light
|
| Type |
Individual Reagent
|
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PhenoVue 577 Lysosomal stain
PhenoVue 577 Lysosomal stain
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Resources
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In living cells, PhenoVue lysosomal stains accumulate in acidic vesicles such as lysosomes, resulting in bright and specific...
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