NGS/PCR cfDNA Solutions

The purification of cell-free DNA (cfDNA) is challenging because of the low concentrations, its instability and the small fragment sizes. Nevertheless the information it delivers is highly valuable for cancer studies and research of prenatal testing using technologies as NGS and ddPCR. The chemagic™ cfDNA kits enable the automated and efficient extraction of cfDNA from 0.5 - 5 ml plasma in 24-well or 96-well format in maximal 2 hours with minimal hands-on time. The chemagic cfDNA kits show equivalent yields as well as purities compared to the manual silica based gold standard kit. Features of the chemagic cfDNA Purification Workflows chemagen developed the chemagic cfDNA kits to isolate cfDNA from 0.2 - 18 ml fresh and frozen plasma derived from EDTA, citrate or other blood collection tubes within 90 - 120 minutes with the chemagic 360 instrument . The chemagic cfDNA kits are based on chemagen technology using M-PVA Magnetic Beads for the isolation of cfDNA. The cfDNA binds to paramagnetic beads, which are magnetically separated from the sample material. During subsequent steps, contaminants are removed and the purified cfDNA is transferred into an elution medium. The automated sample processing by the chemagic 360 instrument excludes cross-contamination and ensures safe handling of infectious sample material. For more information, please download the technical note about automated cfDNA purification. For research use only. Not for use in diagnostic procedures.

Learn what fast and easy DNA analysis assays are available for the LabChip™ GX Touch Nucleic Acid Analyzer.

The success of sensitive downstream genomic applications such as qPCR depend on robust and reliable upstream sample preparation, which often includes proper sample/tissue homogenization and nucleic acid extraction. As the lab scales their sample throughput and their need for consistency in complex workflows, many have integrated automation-enabled protocols into their workflows. In this application note, a semi-automated workflow for nucleic acid extraction from liver tissue is presented, which leverages the Omni Bead Ruptor Elite bead mill homogenizer with the chemagic 360 nucleic acid extractor, with optional use of a JANUS automated liquid handling workstation.

LabChip® RNA analysis provides a fast and easy way to quantitate RNA samples ranging in size from 100 to 6,000.

This application note describes the automated purification of microRNA using the chemagic 360 instrument. The app note demonstrates efficient isolation of miRNA, suitable for downstream applications in genomics and transcriptomics research.

Biobanking core laboratories and biospecimen repositories are facing the demanding task to extract high-quality DNA and RNA for long-term storage from diverse sample materials like whole blood, buffy coats, and fresh, frozen or fixed tissues. To fulfill the needs of sensitive genetic research applications such as whole genome sequencing, real-time and digital PCR, and methylation analysis, the extraction of high yields of ultra-pure nucleic acids is an essential initial step. The purity of nucleic acids is especially critical when it comes to long-term storage of the extracted DNA and RNA samples. We offer full workflow solutions optimized for biobanking and biorepository demands, including high-throughput DNA and RNA isolation solutions, liquid handling robotics, and nucleic acid quality control and analysis. With its exhaustive offering of instrumentations and kits, we are your experienced biobanking application support from nucleic acid extraction to data. For research use only. Not for use in diagnostic procedures.

Studies of transcriptome from whole blood are often limited due to the instability of RNA caused by nucleases and temperature after sample collection, storage, and a suboptimal isolation. In this application note, we show the successful stabilization of RNA by the S-Monovette® RNA Exact tubes from SARSTEDT® in combination with automated RNA isolation with the chemagic™ Total RNA 9k Kit H24 (CMG-1084-S) on the chemagic™ 360 instrument. High-quality RNA was extracted after up to seven days stored at room temperature and up to 14 days stored at 4 °C. For more information, please download the technical note about automated RNA purification. For research use only. Not for use in diagnostic procedures. Revvity is a trademark of Revvity, Inc. All other trademarks are the property of their respective owners.

Transcriptome analysis from whole blood is an interesting segment of research, but also difficult. RNA quality and yields are influenced by nucleases, inappropriate temperatures, and de novo syntheses after sample collection and storage and have an immediate effect on gene expression analyses. The use of blood collection tubes simplifies this application enormously by stabilizing RNA during sample collection and storage. Here, we show the automated isolation of high-quality RNA with the chemagic™ Total RNA Kit H24 on the chemagic 360 instrument from whole blood stored in PAXgene® Blood RNA Tubes. Samples were stored for several days at room temperature and 4 °C, and high-quality RNA was obtained without any inhibition in RT-PCR assays. For more information, please download the technical note about automated RNA purification from PAXgene® Blood RNA Tubes. For research use only. Not for use in diagnostic procedures. Revvity is a trademark of Revvity, Inc. All other trademarks are the property of their respective owners.

Precision biologics are playing an increasingly powerful role as part of therapeutic strategies such as monoclonal antibodies, bispecific and multispecific antibodies, recombinant proteins, vaccines, and targeted next-generation cell and gene therapies. Harnessing large molecules to create safe and effective therapies is a complex and expensive undertaking. So we help scientists like you develop and streamline your entire biologics workflow, helping you overcome the challenges to bringing consistent, high quality, biological medicines to market – faster than ever before. Explore our solutions and technologies that cover areas of: Biomolecular discovery Biologics characterization Investigating immune function Manufacturing and QA/QC Safety and efficacy

This brochure gives overview about flexible automated workflows for DNA/RNA extraction with the chemagic 360 instrument.

The chemagic brochure gives a complete overview about the chemagic instruments and the corresponding chemagic kits for different application fields like genetic testing, biobanking, oncology research, translational research, and infectious disease research.

Advancing cfDNA analysis: Research insights from the chemagic™ cfDNA Extraction Kit for Mimix HD917 cfDNA Reference Standard The analysis of cell-free DNA (cfDNA) in human plasma is a groundbreaking approach in molecular screening, particularly for detecting genetic alterations in cancer research. This application note explores the compatibility of the Revvity´s Mimix HD917 cfDNA Reference Standard with the chemagic cfDNA 5k Kit (CMG-134), highlighting its significance in enhancing liquid biopsy assays. Research Objectives This study aims to evaluate: The extraction efficiency of cfDNA using the chemagic cfDNA Kit compared to traditional methods. The integrity of cfDNA fragment profiles and allelic frequencies post-extraction. Methodology The research involved extracting cfDNA from a 2 mL sample of the HD917 reference standard using two different extraction methods. The focus was on: Extraction Efficiency: Measuring the yield of cfDNA obtained from each method. Fragment Size Analysis: Assessing the length profiles of cfDNA to ensure compatibility with downstream applications. Conclusion This study underscores the importance of using high quality cfDNA extraction methods in oncology research. The compatibility of the Mimix HD917 cfDNA Reference Standard with the chemagic cfDNA 5K Kit not only enhances extraction efficiency but also preserves the integrity of cfDNA for accurate genetic analysis. These findings support the advancement of non-invasive diagnostic techniques in cancer detection and monitoring. For detailed insights and data, download the full application note or contact us for further information. For research use only. Not for use in diagnostic procedures.

With automation being increasingly employed in laboratories performing nucleic acid purification, a more consistent quality of nucleic acids is generally expected. A high quality of should also be obtainable with automation to meet requirements of demanding downstream assays such as NGS, long read sequencing, and MLPA, while ensuring long-term storage stability. However, differences in mechanical processes and kit chemistries between commercial automation platforms may result in varying qualities of nucleic acid which can impact downstream results. Here, we evaluated two systems of magnetic bead-based automated nucleic acid purification using blood samples. High molecular weight DNA acid quality was assessed in terms of yield and purity. Integrity of DNA as seen by the extraction efficiency of HMW DNA was also assessed. Extraction performance from blood based on DNA yield, purity, and integrity (DNA length) was compared between two automated nucleic acid extraction systems. The chemagic™ 360 instrument extracted pure DNA at higher yields and integrity with chemagic™ DNA Blood 400 Kit H96 (CMG-1091) or chemagic DNA Blood 4k Kit H24 (CMG-1074). A greater HMW DNA extraction efficiency was obtained with the chemagic 360 instrument that can improve performance in long-read sequencing as well as long-term storage stability. For research use only. Not for use in diagnostic procedures.

Revvity´s application note on SARS-CoV-2 detection in wastewater provides a crucial overview of the methods used to monitor and analyze the presence of SARS-CoV-2 in sewage systems. This surveillance serves as an early warning system to detect and track the spread of the virus,

Dried blood spots (DBS) are widely utilized in newborn screening, large population-based surveys, and biobanking due to their minimal blood volume requirements, ease of collection, and convenient transportation and storage. However, extracting high-quality DNA from DBS for next-generation sequencing (NGS) has posed significant challenges. This application note presents Revvity’s optimized workflow for targeted sequencing of DNA extracted from DBS, which addresses these challenges effectively. The workflow begins with the DBS Puncher™ instrument, which punches samples into 96-well microtitration plates. DNA is then isolated using the chemagic™ DNA CS200 DNA Kit on the chemagic™ 360 instrument, capable of processing up to 1,000 samples per day. Central to this process is chemagen™ Technology, which employs an automated magnetic separation procedure to isolate high molecular weight (HMW) genomic DNA (gDNA) without denaturing or fragmenting nucleic acids. Quality control is ensured using the LabChip® GX Touch™ nucleic acid analyzer. The DNA is sheared to a target size of 150-200 bp, and libraries are prepared using Agilent® SureSelect® XT kits. The entire process is automated on the Sciclone® G3 NGSx workstation, and sequencing is performed on an Illumina® NovaSeq® sequencer. The results demonstrate high-quality sequencing data with over 99% of reads mapped and low duplicate read percentages, confirming the effectiveness of Revvity’s workflow in producing pure, high-quality DNA suitable for NGS analysis. This optimized workflow facilitates the use of DBS in various research and biobanking applications, overcoming previous limitations in DNA extraction and sequencing. For research use only. Not for use in diagnostic procedures.

Discover the Future of Cancer Research In the realm of oncology, liquid biopsies are revolutionizing how we study and analyze cancer. This case study explores advancements in the extraction and analysis of cell-free DNA (cfDNA), offering essential insights for precision medicine research. By addressing the challenges associated with cfDNA extraction, researchers can enhance the reliability and effectiveness of their cancer studies. What You’ll Learn: The Power of Liquid Biopsies: Understand how cfDNA offers real-time insights into tumor analysis, enabling insightful view in tumor research Challenges in cfDNA Extraction: Explore the common hurdles faced in cfDNA extraction, including low concentration, contamination, and fragmentation. Expert Insights: Gain valuable perspectives from Professor Niels Pallisgaard of Zealand University Hospital, who shares his experiences and findings in utilizing chemagic technology for cancer research. Optimized Workflow: Learn about the streamlined cfDNA extraction process that enhances efficiency and reliability in research settings. Why This Case Study Matters As cancer research advances, the need for precise and reliable research tools becomes paramount. This case study not only highlights the technological innovations in cfDNA extraction but also emphasizes their impact cancer research. Get Access Now To read the full case study and unlock the potential of chemagic technology in precision oncology, please fill out the form below. Join the forefront of cancer research and discover how these advancements can transform oncology research. For research use only. Not for use in diagnostic procedures.

The use of CRISPR-Cas9 tools for gene editing has opened new possibilities for the genetic optimization of biological systems with biotechnological or therapeutic applications. One area of interest is the design of enhanced expression platforms and the generation of new host cell lines that have the required phenotypic advantages and performance for use in biopharmaceutical manufacturing. However, the long-term success of generating new host cells depends greatly on the ability to perform multiplexed gene editing with high efficiency and the deployment of suitable quality control methodologies to accurately evaluate all editing outcomes. In this whitepaper, we demonstrate Revvity’s workflow exemplifying the successful simultaneous knock-out of multiple endogenous loci in CHO cells and show the absence of the targeted proteins in several of the selected clones, proving the efficiency of the multiplexed gene editing approach. It’s also shown that simultaneous knock-out of multiple loci using CRISPR-Cas9 and multiple gRNAs is an effective strategy to enhance CHO cell protein production levels. In collaboration with Cergentis, read more about the data generated including: Gene copy number analysis and screening for gene editing efficiency Generation and characterization of an edited pool Isolation and phenotypic validation of edited clones Targeted Locus Amplification For research use only. Not for use in diagnostic procedures.

Fetal Rhesus Factor D (RhD) genotyping is typically performed through non-invasive prenatal testing (NIPT) and is important in identifying RhD incompatibility between mother and child. NIPT avoids risks associated with invasive procedures such as amniocentesis or chorionic villus sampling and is performed with a simple blood draw to analyze cell free fetal DNA (cffDNA) present in the mother’s blood. The FetoGnost® Kit RHD from Ingenetix allows rapid, sensitive, and non-invasive fetal RhD genotyping of samples purified from maternal plasma of RhD-negative pregnant women, based on real-time PCR technology. The chemagic™ automated nucleic acid isolation technology has been previously demonstrated to provide high and consistent yields of cell free DNA (cfDNA) from plasma when compared to manual column procedures. Here, compatibility of the FetoGnost Kit RHD with extracted cffDNA obtained with the chemagic™ 360 instrument was tested using RhD-positive or -negative plasma from 36 blood donors. For research use only. Not for use in diagnostic procedures. *Revvity does not endorse or make recommendations with respect to research, medication, or treatments. All information presented is for informational purposes only and is not intended as medical advice. For country specific recommendations, please consult your local health care professionals. Revvity is a trademark of Revvity, Inc. All other trademarks are the property of their respective owners.

Flyer showcasing Revvity's solutions for key steps of your single-cell sequencing journey.

A major challenge of using Whole-Genome Sequencing (WGS) for high-throughput genomic analysis is the amount of time required to perform the entire procedure from DNA isolation from the primary samples to analysis of the sequencing results. Fast next-generation sequencing requires the isolation of high-quality nucleic acids in a very short time frame and the optimization of the downstream pipeline. In the application note below, we describe how fast DNA isolation from high volumes of blood coupled to modifications to an Illumina® HiSeq®2500 instrument and optimization of base-calling and variant detection using Isaac software v1 can decrease the time from sample preparation to sequence analysis to less than 35 hours. Revvity’s chemagic™ research instrumentation and kits offer optimal solutions for high-throughput DNA extraction from a wide variety of sample types by providing: High DNA yields– e.g., up to 50 µg DNA/ml blood Capacity to handle large volumes of primary samples – more DNA available for testing Fast processing times– e.g., 24 blood samples in 50 min Tested sample handling technology – lower cross-contamination occurrences Full or partial automated systems – less hands-on time during the nucleic acid purification procedure High levels of DNA integrity – up to 200 kb fragments suitable for the most complex genomic analyses Long-term stable nucleic acids – ideal for biobanking and clinical research applications For more information, please download the application note or contact us. For research use only. Not for use in diagnostic procedures.

Plants are essential to our diet and ecosystem; researchers are constantly working to better understand their genetic makeup and diversity. Traditional enzymatic lysis methods to extract plant DNA are time-consuming and can be inconsistent due to the toughness of their cell walls. Physical disruption via bead mill homogenization can be an efficient alternative to both rudimentary enzymatic and manual methods. We review the workflow and determine yields from staple crops such as corn, rice, and wheat in this application note. Automation-enabled solutions help scale throughput while providing consistent and efficient sample preparation and nucleic acid extraction from plant material.

Corn is an important stable crop, not only as a food source for humans and livestock, but also biofuel as well other non-food products. This versability makes corn an importance resource and topic of research globally. Within agricultural science and sustainability, labs are hard at work better understanding the genetic mechanisms behind healthy growth and environmental adaptability, whether in response to factors such as climate change, availability of arable land, susceptibility to disease. With the adoption of high-throughput sequencing assessing plant transcriptomics, labs require sample prep methods that reliably allows the extraction of RNA with high integrity for downstream assays, which can be challenging due to thick polysaccharide cell walls and the layers inherent to certain sample types. Semi-automated tabletop bead mills allow for repeatability only afforded by automation-enabled techniques that feed into automated nucleic acid extractions that scales as your throughput increases. For research use only. Not for use in diagnostic procedures.

The processing of human and animal tissue samples from experimental studies for downstream molecular analysis is a cornerstone of pre-clinical research across multiple disciplines spanning agrigenomics and disease research. Within animal anatomy, soft tissues are a vital component of not only organ-specific research, but also for providing insights into drug discovery or development work. Genomic workflows, including the critical upfront sample prep for efficient nucleic acid isolation of DNA and/or RNA is an important application area. Though there are multiple methods for tissue disaggregation and subsequent sample homogenization, bead mill homogenization is particularly effective for its ability to provide consistent and uniform homogenates via mechanical forces and cell disruption. In the same vein, the incorporation of automation into workflows reduces the risk of introducing human error and inconsistencies between samples. In this application note, an automation-enabled workflow for bead mill homogenization into an automated nucleic acid extractor is described to observe run-to-run reproducibility between the groups of samples extracted for DNA on different days using the same method.

Benefit of an automated cfDNA analysis workflow The presence of double stranded, circulating cell free DNA (cfDNA) in blood plasma was discovered more than 70 years ago. Soon after, a link between cfDNA and leukemia as well as autoimmune disease was found. Recently, the development of cfDNA-based prenatal genetic testing and the realization that cfDNA can be used to detect and monitor tumor specific mutations in cancer patients has increased the interest in cfDNA analyses. Read the application note to review the results and how much time you could save by automating your cfDNA analysis workflow using Revvity’s automated solution including blood fractionation, cfDNA isolation, library prep, QC, and quantitation.

Here Revvity and Illumina describe a streamlined, automated workflow from DNA extraction to sequence-ready libraries for metagenomic shotgun sequencing resulting in high quality data for species identification and functional profiling.