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HTRF Human FMRP Detection Kit, 500 Assay Points

The Human FMRP kit is designed for the rapid detection of human FMRP in cell lysates.

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Feature	Specification
Application	Protein Quantification
Sample Volume	10 µL

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The Human FMRP kit is designed for the rapid detection of human FMRP in cell lysates.

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Product Variants

Part #:

63ADK038PEG

List Price

USD 1,082.64

Part #:

63ADK038PEH

List Price

USD 12,243.50

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product information

Overview

FMRP (Fragile X Mental Retardation Protein) is a brain protein known to play an important role in normal cognitive development and female reproductive function. fmr1 gene silencing has been correlated to fragile X syndrome onset triggering severe learning deficits or mental retardation. Mutations of the fmr1 gene can also be associated to intellectual disability, premature ovarian failure, autism, Parkinson's disease, developmental delays, and other cognitive deficits. The human FMRP kit enables the fast monitoring of FMRP produced by cells.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Biomarkers
Target Species	Human
Technology	TR-FRET
Therapeutic Area	Neuroscience Rare Diseases
Unit Size	500 Assay Points

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How it works

Assay principle

Human FMRP is measured using a sandwich immunoassay involving two specific anti-FMRP antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the FMRP present in the sample.

Assay protocol

The human FMRP assay features a two-plate assay protocol, where cells are plated, stimulated, and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of Human FMRP. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.