Chemiluminescent Blotting Substrates
Western blotting at lightning performance
Chemiluminescent substrates are important tools in the detection of proteins and nucleic acids. Essentially, chemiluminescence blot detection starts when the primary antibody recognizes and binds to the target protein or nucleic acid immobilized on a membrane. This is followed by a secondary antibody labeled with an enzyme such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). A chemiluminescent substrate for the particular enzyme is then applied to the blot causing a light emitting reaction which is then detected, signaling the presence of the target.
Western Lightning™ ONE
Our premier line of premixed one-component chemiluminescent HRP substrates, are available with protein detection sensitivity from picogram to low femtogram to cover all your detection needs. Using a proprietary technology, our premixed solution enables greater experimental consistency by avoiding pipetting errors and possible contamination.
Western Lightning™
Our range of two-component chemiluminescent HRP substrates offers protein detection at varying sensitivities to fit your needs.
Use our chemiluminescent substrates for sensitive and consistent detection of proteins in a wide range of biochemistry, immunogenetic, and molecular biology applications.
Discover our chemiluminescent blotting substrates
| Product | Format | Enzyme | Blotting method (Target) | Recommended membrane |
|---|---|---|---|---|
| Western Lightning™ ONE | One bottle – premixed, no pipetting required | HRP | Western (protein) | Protran™ nitrocellulose membrane |
| Western Lightning™ | Two bottle – requires mixing | HRP | Western Protein | Protran™ nitrocellulose membrane |
For research use only. Not for use in diagnostic procedures.
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When proteins are low and antibodies are precious, our extreme-level pre-mixed one-component chemiluminescent horseradish peroxidase (HRP) substrate with low antibody consumption is the right choice. Western Lightning™ ONE Femto Ultra can detect low-femtogram protein levels in western blots with superior signal intensity.
A chemiluminescent substrate for western blotting with highest sensitivity to detect proteins in the low femtogram range. Ideal for conservation of precious samples.
Our one-component chemiluminescent horseradish peroxidase (HRP) substrates are ideal for routine analysis detection of abundant proteins. Western Lightning™ ONE Pico can detect mid-picogram protein levels in western blots and provides low background for a high signal to noise ratio.
A versatile, mid-level pre-mixed, one-component chemiluminescent horseradish peroxidase (HRP) substrate appropriate for a wide range of antibody dilutions, Western Lightning™ ONE Femto can detect mid-femtogram protein levels in western blots with a high signal intensity.
An enhanced chemiluminescent substrate for western blotting, with the sensitivity and signal-to-noise ratio needed to achieve consistent, reliable results. For use with either PVDF or nitrocellulose membranes.
An enhanced chemiluminescent substrate for western blotting, with the sensitivity to detect proteins in the low picogram range. With a long-lasting luminescent signal for repeated exposures, the unique non-toxic formulation provides an excellent dynamic range.
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