Revvity Sites Globally

Select your location.

*e-commerce not available for this region.

Australia

Austria

Belgium

Brazil *

Canada

China *

Denmark

Finland

France

Germany

Hong Kong *

India *

Ireland

Italy

Japan *

Luxembourg

Mexico *

Netherlands

Norway

Philippines *

Republic of Korea *

Singapore *

Spain

Sweden

Switzerland

Thailand *

United Kingdom

United States

HTRF pAb Anti-Phospho-ATF2 (Thr69/71) Eu-Conjugate, 500 Assay Points

PAb Anti-phospho ATF2-Eu cryptate enables the rapid detection of ATF2 phosphorylation on Thr69/71 in a biochemical kinase assay format.

View product information

Feature	Specification
Application	Biochemical Enzymatic Assay

Request More Information

PAb Anti-phospho ATF2-Eu cryptate enables the rapid detection of ATF2 phosphorylation on Thr69/71 in a biochemical kinase assay format.

View product information

Product Variants

Part #:

61P12KAE

List Price

USD 915.62

Part #:

61P12KAZ

List Price

USD 2,417.53

For research use only. Not for use in diagnostic procedures.

Product information

Overview

The Anti-phospho ATF2-Eu cryptate is a labeled polyclonal antibody enabling the detection of ATF2 when singly phosphorylated at Ser 71 or dually phosphorylated at Thr69/71. It does not detect the non-phosphorylated ATF2. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.

Specifications

Other Specifications

Application	Biochemical Enzymatic Assay
Brand	HTRF
Detection Modality	HTRF
Molecular Modification	Phosphorylation
Product Group	Fluorescent Reagent
Shipping Conditions	Shipped in Dry Ice
Target Class	Kinase
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Inflammation Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	500 Assay Points

Video gallery

How it works

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

HTRF PAb Anti-phospho ATF2-Eu cryptate 1.svg

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Anti-phospho ATF2-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.