The LANCE® Ultra c-Myc Detection Kit is designed for detection and quantitation of c-Myc peptide tag in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Feature | Specification |
---|---|
Application | Protein-Protein Interaction |
Dynamic Range | 46.1- 300,000 nM |
Limit of Detection | 46.1 nM |
Limit of Quantification | 235.8 nM |
Sample Volume | 5 µL |
The LANCE® Ultra c-Myc Detection Kit is designed for detection and quantitation of c-Myc peptide tag in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The LANCE Ultra c-Myc Detection Kit is designed for detection and quantitation of c-Myc peptide tag in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with an acceptor fluorophore [ULight™ dye]. A “tracer” analyte is biotinylated and binds Streptavidin labeled with donor Eu-chelate. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. In the presence of sufficient non-biotinylated analyte this emission will be diminished.
The c-myc polypeptide tag is derived from the c-myc gene. The tag can be used during affinity chromatography and to separate wild-type protein from recombinant protein. The c-myc tag can be used in indirect detection assays for which there is no antibody for the target.
Application |
Protein-Protein Interaction
|
---|---|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Modality |
LANCE
|
Dynamic Range |
46.1- 300,000 nM
|
Limit of Detection |
46.1 nM
|
Limit of Quantification |
235.8 nM
|
Product Group |
Kit
|
Sample Volume |
5 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
c-Myc
|
Target Class |
Binding Assay
|
Target Species |
Human
|
Technology |
TR-FRET
|
Therapeutic Area |
Oncology
|
Unit Size |
500 Assay Points
|
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