PhenoVue Cell Painting Kits

In the ground-breaking realm of gene therapy, adeno-associated virus (AAV) vectors are revolutionizing our approach to treating ocular diseases. In this white paper, 'AAV vectors: Pursuing a one-time therapy for ocular diseases,' we discuss with Professor Dominik Fischer, a renowned Ophthalmic Surgeon and Professor of Ophthalmology, the remarkable potential and challenges of developing AAV gene therapies in ocular disorders. Learn about the critical challenges of ensuring long-term gene expression, the durability of these therapies, and innovative strategies to enhance treatment effectiveness while managing potential toxicity. This white paper is an essential read for anyone interested in the latest advancements in gene therapy and its transformative impact on ocular health.

This whitepaper delves into key concepts, strategies, and best practices for leveraging high- content imaging (HCI) methods such as cell painting to explore gene-regulated responses and correlate cellular phenotypes to genome profiles. Learn how to implement effective screening workflows, acquire and analyze phenotypic data, and overcome the challenges of handling large datasets. Equip your team with the knowledge to harness the full potential of functional genomics for therapeutic intervention and groundbreaking biological discoveries.

Phenotypic Drug Discovery Re-Imagined Cell Painting is a phenotypic screening method and a powerful application of high-content screening technology which combines cell and computational biology to elucidate the behavior of cells under the influence of perturbagens, such as chemical compounds, drugs, genes, or other entities. Download our white paper to learn about the origins of the Cell Painting technique, its potential impact on the drug discovery paradigm, together with practical hints and tips for success.

Cell painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens. However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis. Download our application note to learn how to: Set up optimal imaging parameters and see the effect of different acquisition modes on compound clustering Easily perform the assay using the dyes provided in the PhenoVue ® cell painting kit Extract and analyze more than 5700 cellular features Visualize multidimensional data using the Signals ™ VitroVivo platform

Cell painting is an imaging-based phenotypic profiling method that measures phenotypic features to characterize the biological responses of cells to chemical and genetic perturbagens. It finds uses in functional genomics, drug discovery, efficacy, toxicity assessment, and in screening for insights into mechanism-of-action. In this infographic, you will learn: What cell painting is, its history, and how it works What you need to get started How to easily perform the assay using our cell painting solutions

With powerful potential for phenotypic drug discovery, cell painting is being rapidly adopted by leading scientists across the globe. However, implementing cell painting assays can present some challenges. To help you avoid some of the common pitfalls, we’ve collated answers from our experts to the top questions that we’ve been hearing from scientists and researchers. You will learn: How to choose the cell model What to look for in labelling reagents Key considerations for image acquisition Recommendations for image analysis Data analysis approaches

This article explores current and future trends in the development and use of genetic payloads, with a focus on some of the advances in gene editing platforms and how these enable more complex editing tasks while reducing the risk of unintended genomic rearrangements. Discover the advancements in CRISPR-Cas9 gene editing, the development of the Pin-point ™ base editing system, and how these technologies are revolutionizing the field.

The PhenoVue™ cell painting kit utilizes six fluorescent dyes to reveal a broad range of cellular morphologies and subtle phenotypic changes. But sometimes, a specific research area, cell model, or stage of drug discovery requires a different combination of dyes. For this, Revvity has developed the PhenoVue multi- organelle kit. This kit offers five distinct probes targeting lipid droplets and lysosomes, with a simplified protocol for fixed cells and limited spectral overlap, enabling you to pinpoint the specific cellular structures you’re interested in. In collaboration with the Institute of Bioorganic Chemistry (IBCH) in Poland, we evaluated phenotypic discrimination using 15 reference compounds. Both the PhenoVue cell painting kit and the multi- organelle kit revealed key differences in phenotypic profiles, demonstrating the unique strengths of each assay. Download our app note to learn more.

Combining CRISPR gene editing and cell painting provides researchers with the ability to correlate genotypes with phenotypes and holds the promise to help understand gene functions in more detail and at greater scale. This application note demonstrates a harmonized workflow for morphological profiling of CRISPR-Cas9-mediated knockouts using cell painting, with simplified feature extraction using the dedicated Cell Painting Building Block in Harmony ® High-Content Analysis software. Workflow for an arrayed CRISPR knockout screen using Cas9 stable cells and Edit-R ® synthetic single guide RNAs Phenotypic analysis of gene knockouts with the PhenoVue ™ Cell Painting JUMP kit and the Opera Phenix ® Plus High-Content Screening system One step solution for cell painting feature extraction in Harmony High-Content Analysis software Phenotypic clustering using Principal Component Analysis and Self-Organizing Maps

Details of PhenoVue Cell Painting JUMP Kit, 1 x 384-wells.

Details of PhenoVue Cell Painting JUMP Kit, 10 x 384-wells.

This is a product information sheet for PhenoVue Cell Painting Kit 1 x 384-Well Plate.

This flyer describes Revvity's PhenoVue cellular imaging reagents.