Phenotypic analysis of CRISPR-Cas9 cell-cycle knockouts using cell painting

Combining CRISPR gene editing and cell painting provides researchers with the ability to correlate genotypes with phenotypes and holds the promise to help understand gene functions in more detail and at greater scale.

This application note demonstrates a harmonized workflow for morphological profiling of CRISPR-Cas9-mediated knockouts using cell painting, with simplified feature extraction using the dedicated Cell Painting Building Block in Harmony^® High-Content Analysis software.

• Workflow for an arrayed CRISPR knockout screen using Cas9 stable cells and Edit-R^® synthetic single guide RNAs
• Phenotypic analysis of gene knockouts with the PhenoVue^™ Cell Painting JUMP kit and the Opera Phenix^® Plus High-Content Screening system
• One step solution for cell painting feature extraction in Harmony High-Content Analysis software
• Phenotypic clustering using Principal Component Analysis and Self-Organizing Maps

For research use only, not for use in diagnostic procedures.