US
Search Results
Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
HTRF IP-One Gq Detection Kit, 1,000 Assay Points
This HTRF kit detects the accumulation of inositol monophosphate, a metabolite produced following phospholipase C activation and used as a proxy of Gq activation
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
HTRF IP-One assays detect the accumulation of inositol monophosphate, a stable downstream metabolite of IP3 induced by activation of a phospholipase C (PLC) cascade. This kit allows direct characterization of all types of compounds acting on Gq-coupled receptors in either adherent or suspension cells.
From high-throughput screening (HTS) to structure-activity relationships (SAR), IP-One has been shown to offer superior benefits over traditional assays
Specifications
| Assay Points |
1000
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped Ambient
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
1,000 Assay Points
|
Video gallery
References
How it works
Assay principle
The kit is based on a competitive format involving a specific antibody labeled with cryptate (donor) and IP1 coupled to d2 (acceptor). Native IP1 produced by cells competes with d2-labeled IP1 for binding to monoclonal anti-IP1 Tb2+ cryptate. LiCl is added to the cell stimulation buffer, causing IP1 to accumulate upon receptor activation.
Assay protocol
The IP-One assay features a streamlined protocol with just two incubation steps: Cell stimulation by the ligand or target compounds IP1 detection using HTRF reagentsThis protocol requires only a single, one-hour incubation period following cell stimulation.
Assay details
Specificity of IP-One Gq assay
IP-One ensures signal specificity, measuring only IP-1, an analyte that is solely produced by the action of the phospholipase enzyme (PLC) and mediated by GPCRs.
In contrast, calcium responses are complex in nature. Calcium release is frequently triggered by non-G protein mechanisms, including Ca2+ permeable channels, calcium pumps, calcium transporters.
Standard curve characteristics
Assay Window, sensitivity, reproducibility and specificity : IP-One makes no concession !
Resources
Whitepaper
An overview of atherosclerosis
Atherosclerosis pathogenesis, cellular actors, and pathways
Atherosclerosis is a common condition in which arteries harden and...
Whitepaper
Discover the benefits of Inositol phosphate assays over calcium flux when studying Gaq signaling
The essential guide to Gq signaling
This White Paper aims to provide you with the information related to the Inositol phosphate...
Guide
Fast track to a successful Gαq assay optimization
2 guides to master the art of optimizing your IP-One assays!
Streamlining IP-One assay optimization:
- A step-by-step approach to...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
How can we help you?
We are here to answer your questions.
